| RRC ID |
44890
|
| 著者 |
Qi H, Wu Q, Abe N, Saiki S, Zhu B, Murata Y, Nakamura Y.
|
| タイトル |
Ascorbic acid synergistically potentiates phloxine b-induced photocytotoxicity in human acute promyelocytic leukemia cells.
|
| ジャーナル |
J Biochem Mol Toxicol
|
| Abstract |
Ascorbic acid (AsA) is known as an antioxidant but concomitantly possesses a pro-oxidant property. Because the impact of AsA on photodynamic therapy response is unclear, we investigated the effect of AsA on photocytotoxicity induced by phloxine B in human acute promyelocytic leukemia HL-60 cells. AsA synergistically enhanced phloxine B-induced photocytotoxic effects, including inhibition of cell proliferation, DNA ladder formation, and caspase-3 activation, whereas AsA itself showed no photocytotoxicity. AsA also enhanced the consumption of the reduced glutathione level compared with the cells treated with phloxine B alone under the light condition. Combination of AsA with phloxine B under the light condition enhanced the phosphorylation of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase (MAPK). These effects were completely cancelled by catalase. These results suggest that AsA synergistically enhances phloxine B-induced photocytotoxicity, possibly through the extracellular oxidative stress-dependent MAPK pathway activation.
|
| 巻・号 |
28(4)
|
| ページ |
167-73
|
| 公開日 |
2014-4-1
|
| DOI |
10.1002/jbt.21549
|
| PMID |
24488945
|
| MeSH |
Antineoplastic Agents / pharmacology*
Apoptosis
Ascorbic Acid / pharmacology*
Caspase 3 / metabolism
Cell Line, Tumor
Cell Proliferation / drug effects
DNA Fragmentation
Drug Synergism
Eosine I Bluish / pharmacology*
Glutathione / metabolism
HL-60 Cells
Humans
JNK Mitogen-Activated Protein Kinases / metabolism
MAP Kinase Signaling System
Oxidative Stress
Phosphorylation
Photosensitizing Agents / pharmacology*
Protein Processing, Post-Translational
p38 Mitogen-Activated Protein Kinases / metabolism
|
| IF |
3.606
|
| 引用数 |
5
|
|
WOS 分野
|
TOXICOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
