| RRC ID |
44892
|
| Author |
Kaneko H, Sasaki H, Honma S, Hayakawa T, Sato M, Yajima Y, Yoshinari M.
|
| Title |
Influence of thin carbonate-containing apatite coating with molecular precursor method to zirconia on osteoblast-like cell response.
|
| Journal |
Dent Mater J
|
| Abstract |
The influence of a thin carbonate-containing hydroxyapatite (CA) coating to tetragonal zirconia polycrystal (TZP) on osteoblastlike cell response was investigated. TZP disks were subjected to blasting and acid etching. Thin CA coatings were deposited by the molecular precursor method (TZP-CA). Initial cell adhesion of mouse osteoblast-like cells MC3T3-E1 was enhanced, and marked progress of actin filaments was observed on TZP-CA compared to on TZP. After 3, 5 or 7 days, cell proliferation on TZP-CA was significantly higher than that on TZP. Alkaline phosphatase activity was slightly lower on TZP-CA than on TZP at 7 days, and no difference was observed at 14 or 21 days. At 28 days incubation, collagenous fibers with mineral precipitants accompanied by phosphorous and amino groups were observed. These results indicate that thin CA coating with molecular precursor method offers promise as a means of enhancing cell response, particularly initial adhesion and proliferation of MC3T3-E1 cells.
|
| Volume |
33(1)
|
| Pages |
39-47
|
| Published |
2014-1-1
|
| DOI |
10.4012/dmj.2013-122
|
| PII |
DN/JST.JSTAGE/dmj/2013-122
|
| PMID |
24492110
|
| MeSH |
Acid Etching, Dental
Actins / physiology
Alkaline Phosphatase / metabolism
Animals
Apatites / chemistry*
Cell Adhesion
Cell Differentiation
Cell Proliferation
Cells, Cultured
Coated Materials, Biocompatible / chemistry*
Materials Testing
Mice
Osteoblasts / physiology*
Surface Properties
Zirconium / chemistry*
|
| IF |
1.359
|
| Times Cited |
7
|
|
WOS Category
|
DENTISTRY, ORAL SURGERY & MEDICINE
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
