RRC ID 44895
Author Tsutsumi K, Saito N, Kawazoe Y, Ooi HK, Shiba T.
Title Morphogenetic study on the maturation of osteoblastic cell as induced by inorganic polyphosphate.
Journal PLoS One
Abstract Since inorganic polyphosphates [poly(P)] have an activity to induce bone differenciation in vitro and in vivo, we examined an effect of poly(P) on organelle by light microscopy and electron microscopy in Murine MC3T3-E1 osteoblastic cells. The MC3T3-E1 cells were ultrastructurally observed to possess morphological characteristics of osteoblasts. Cells cultured with poly(P) were strongly stained with an anti-collagen type I antibody but not in those cultured without poly(P). Ultrastructural analysis of cells cultured with poly(P) revealed a well-developed Golgi apparatus, swollen and elongated rough endoplasmic reticulum, large mitochondria and many coated pits. Since MC3T3-E1 cells can be transformed from a resting phase to an active blastic cell phase after supplementation with poly(P), it implies that poly(P) can be an effective material for bone regeneration.
Volume 9(2)
Pages e86834
Published 2014-1-1
DOI 10.1371/journal.pone.0086834
PII PONE-D-13-34148
PMID 24498284
PMC PMC3911941
MeSH Animals Cell Differentiation / drug effects* Cell Line Collagen Type I / metabolism Cytoplasm / drug effects Cytoplasm / metabolism Cytoplasm / ultrastructure Mice Microscopy, Electron, Transmission Microscopy, Phase-Contrast Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / ultrastructure Osteogenesis / drug effects* Osteopontin / metabolism Polyphosphates / pharmacology* Time Factors Vacuoles / drug effects Vacuoles / ultrastructure
IF 2.74
Times Cited 25
Human and Animal Cells MC3T3-E1(RCB1126)