| RRC ID |
44909
|
| 著者 |
Higuchi Y, Kawai K, Yamamoto M, Kuronuma M, Ando Y, Katano I, Nakamura M, Suemizu H.
|
| タイトル |
Novel enhanced green fluorescent protein-expressing NOG mouse for analyzing the microenvironment of xenograft tissues.
|
| ジャーナル |
Exp Anim
|
| Abstract |
The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which strongly expresses enhanced green fluorescent protein (EGFP; PgkEGFP-NOG), especially in the liver, kidney, gastrointestinal tract, and testis. Because the host tissues expressed EGFP, xenotransplanted human cancer cells were clearly identified as EGFP-negative colonies in PgkEGFP-NOG mice. Immunohistochemical analysis revealed that EGFP-expressing stromal tissues formed a complicated tumor microenvironment within xenograft tissues. Moreover, a similar microenvironment was observed in human iPS cell-derived teratomas. Collectively, these results indicated that a suitable microenvironment is essential for the growth and maintenance of xenotransplanted cells and that PgkEGFP-NOG mice represent a useful animal model for analyzing the mechanisms of microenvironment formation.
|
| 巻・号 |
63(1)
|
| ページ |
55-62
|
| 公開日 |
2014-1-1
|
| DOI |
10.1538/expanim.63.55
|
| PII |
DN/JST.JSTAGE/expanim/63.55
|
| PMID |
24521863
|
| PMC |
PMC4160926
|
| MeSH |
Animals
Colorectal Neoplasms / pathology*
Gene Expression
Green Fluorescent Proteins / genetics*
HCT116 Cells
Heterografts
Humans
Immunohistochemistry
Induced Pluripotent Stem Cells / pathology*
Mice
Mice, Inbred NOD*
Models, Animal
Neoplasm Transplantation*
Teratoma / pathology*
Tumor Microenvironment*
|
| IF |
1.574
|
| 引用数 |
1
|
|
WOS 分野
|
VETERINARY SCIENCES
ZOOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063) |
