論文 - 詳細
| RRC ID | 44909 |
|---|---|
| 著者 | Higuchi Y, Kawai K, Yamamoto M, Kuronuma M, Ando Y, Katano I, Nakamura M, Suemizu H. |
| タイトル | Novel enhanced green fluorescent protein-expressing NOG mouse for analyzing the microenvironment of xenograft tissues. |
| ジャーナル | Exp Anim |
| Abstract |
The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which strongly expresses enhanced green fluorescent protein (EGFP; PgkEGFP-NOG), especially in the liver, kidney, gastrointestinal tract, and testis. Because the host tissues expressed EGFP, xenotransplanted human cancer cells were clearly identified as EGFP-negative colonies in PgkEGFP-NOG mice. Immunohistochemical analysis revealed that EGFP-expressing stromal tissues formed a complicated tumor microenvironment within xenograft tissues. Moreover, a similar microenvironment was observed in human iPS cell-derived teratomas. Collectively, these results indicated that a suitable microenvironment is essential for the growth and maintenance of xenotransplanted cells and that PgkEGFP-NOG mice represent a useful animal model for analyzing the mechanisms of microenvironment formation. |
| 巻・号 | 63(1) |
| ページ | 55-62 |
| 公開日 | 2014-1-1 |
| DOI | 10.1538/expanim.63.55 |
| PII | DN/JST.JSTAGE/expanim/63.55 |
| PMID | 24521863 |
| PMC | PMC4160926 |
| MeSH | Animals Colorectal Neoplasms / pathology* Gene Expression Green Fluorescent Proteins / genetics* HCT116 Cells Heterografts Humans Immunohistochemistry Induced Pluripotent Stem Cells / pathology* Mice Mice, Inbred NOD* Models, Animal Neoplasm Transplantation* Teratoma / pathology* Tumor Microenvironment* |
| IF | 1.574 |
| 引用数 | 1 |
| WOS 分野 | VETERINARY SCIENCES ZOOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |