RRC ID 4492
Author Yamagishi K, Sugiyama M, Kaneko Y, Nishizawa M, Harashima S.
Title Construction and characterization of single-gene chromosomes in Saccharomyces cerevisiae.
Journal J. Biosci. Bioeng.
Abstract Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2-0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.
Volume 106(6)
Pages 563-7
Published 2008-12
DOI 10.1263/jbb.106.563
PII S1389-1723(09)70008-8
PMID 19134552
MeSH Blotting, Southern Chromosomes, Fungal* Electrophoresis, Agar Gel Genes, Fungal* Saccharomyces cerevisiae / genetics*
IF 2.015
Times Cited 3
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Yeast ?