RRC ID |
4492
|
Author |
Yamagishi K, Sugiyama M, Kaneko Y, Nishizawa M, Harashima S.
|
Title |
Construction and characterization of single-gene chromosomes in Saccharomyces cerevisiae.
|
Journal |
J Biosci Bioeng
|
Abstract |
Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2-0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.
|
Volume |
106(6)
|
Pages |
563-7
|
Published |
2008-12-1
|
DOI |
10.1263/jbb.106.563
|
PII |
S1389-1723(09)70008-8
|
PMID |
19134552
|
MeSH |
Blotting, Southern
Chromosomes, Fungal*
Electrophoresis, Agar Gel
Genes, Fungal*
Saccharomyces cerevisiae / genetics*
|
IF |
2.366
|
Times Cited |
3
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
Resource |
Yeast |
|