RRC ID 44929
著者 Ishikawa K, Ishikawa A, Shoji Y, Imai T.
タイトル A genotoxic stress-responsive miRNA, miR-574-3p, delays cell growth by suppressing the enhancer of rudimentary homolog gene in vitro.
ジャーナル Int J Mol Sci
Abstract MicroRNA (miRNA) is a type of non-coding RNA that regulates the expression of its target genes by interacting with the complementary sequence of the target mRNA molecules. Recent evidence has shown that genotoxic stress induces miRNA expression, but the target genes involved and role in cellular responses remain unclear. We examined the role of miRNA in the cellular response to X-ray irradiation by studying the expression profiles of radio-responsive miRNAs and their target genes in cultured human cell lines. We found that expression of miR-574-3p was induced in the lung cancer cell line A549 by X-ray irradiation. Overexpression of miR-574-3p caused delayed growth in A549 cells. A predicted target site was detected in the 3'-untranslated region of the enhancer of the rudimentary homolog (ERH) gene, and transfected cells showed an interaction between the luciferase reporter containing the target sequences and miR-574-3p. Overexpression of miR-574-3p suppressed ERH protein production and delayed cell growth. This delay was confirmed by knockdown of ERH expression. Our study suggests that miR-574-3p may contribute to the regulation of the cell cycle in response to X-ray irradiation via suppression of ERH protein production.
巻・号 15(2)
ページ 2971-90
公開日 2014-2-20
DOI 10.3390/ijms15022971
PII ijms15022971
PMID 24566139
PMC PMC3958894
MeSH 3' Untranslated Regions Cell Cycle Checkpoints Cell Cycle Proteins / antagonists & inhibitors Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Line, Tumor Cell Proliferation / radiation effects DNA Damage / radiation effects Down-Regulation Humans MicroRNAs / genetics MicroRNAs / metabolism* RNA Interference RNA, Small Interfering / metabolism Transcription Factors / antagonists & inhibitors Transcription Factors / genetics Transcription Factors / metabolism* X-Rays
IF 4.556
引用数 15
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, MULTIDISCIPLINARY
リソース情報
ヒト・動物細胞 HeLa NB1RGB(RCB0222)