RRC ID |
44929
|
著者 |
Ishikawa K, Ishikawa A, Shoji Y, Imai T.
|
タイトル |
A genotoxic stress-responsive miRNA, miR-574-3p, delays cell growth by suppressing the enhancer of rudimentary homolog gene in vitro.
|
ジャーナル |
Int J Mol Sci
|
Abstract |
MicroRNA (miRNA) is a type of non-coding RNA that regulates the expression of its target genes by interacting with the complementary sequence of the target mRNA molecules. Recent evidence has shown that genotoxic stress induces miRNA expression, but the target genes involved and role in cellular responses remain unclear. We examined the role of miRNA in the cellular response to X-ray irradiation by studying the expression profiles of radio-responsive miRNAs and their target genes in cultured human cell lines. We found that expression of miR-574-3p was induced in the lung cancer cell line A549 by X-ray irradiation. Overexpression of miR-574-3p caused delayed growth in A549 cells. A predicted target site was detected in the 3'-untranslated region of the enhancer of the rudimentary homolog (ERH) gene, and transfected cells showed an interaction between the luciferase reporter containing the target sequences and miR-574-3p. Overexpression of miR-574-3p suppressed ERH protein production and delayed cell growth. This delay was confirmed by knockdown of ERH expression. Our study suggests that miR-574-3p may contribute to the regulation of the cell cycle in response to X-ray irradiation via suppression of ERH protein production.
|
巻・号 |
15(2)
|
ページ |
2971-90
|
公開日 |
2014-2-20
|
DOI |
10.3390/ijms15022971
|
PII |
ijms15022971
|
PMID |
24566139
|
PMC |
PMC3958894
|
MeSH |
3' Untranslated Regions
Cell Cycle Checkpoints
Cell Cycle Proteins / antagonists & inhibitors
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism*
Cell Line, Tumor
Cell Proliferation / radiation effects
DNA Damage / radiation effects
Down-Regulation
Humans
MicroRNAs / genetics
MicroRNAs / metabolism*
RNA Interference
RNA, Small Interfering / metabolism
Transcription Factors / antagonists & inhibitors
Transcription Factors / genetics
Transcription Factors / metabolism*
X-Rays
|
IF |
4.556
|
引用数 |
15
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CHEMISTRY, MULTIDISCIPLINARY
|
リソース情報 |
ヒト・動物細胞 |
HeLa
NB1RGB(RCB0222) |