| RRC ID |
4492
|
| 著者 |
Yamagishi K, Sugiyama M, Kaneko Y, Nishizawa M, Harashima S.
|
| タイトル |
Construction and characterization of single-gene chromosomes in Saccharomyces cerevisiae.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2-0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.
|
| 巻・号 |
106(6)
|
| ページ |
563-7
|
| 公開日 |
2008-12-1
|
| DOI |
10.1263/jbb.106.563
|
| PII |
S1389-1723(09)70008-8
|
| PMID |
19134552
|
| MeSH |
Blotting, Southern
Chromosomes, Fungal*
Electrophoresis, Agar Gel
Genes, Fungal*
Saccharomyces cerevisiae / genetics*
|
| IF |
2.366
|
| 引用数 |
3
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 酵母 |
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