| RRC ID |
44944
|
| Author |
Odkhuu E, Koide N, Tsolmongyn B, Jambalganiin U, Naiki Y, Komatsu T, Yoshida T, Yokochi T.
|
| Title |
Involvement of redox balance in in vitro osteoclast formation of RAW 264.7 macrophage cells in response to LPS.
|
| Journal |
Innate Immun
|
| Abstract |
Here we report that LPS induces osteoclast (OC) formation in murine RAW 264.7 macrophage cells in RPMI-1640 medium but not in α-minimum essential medium (α-MEM) as the original culture medium. LPS-induced OC formation in both media was examined to clarify the differential response. Receptor activator of NF-κB ligand induced OC formation in either α-MEM or RPMI-1640 medium. However, LPS-induced OC formation in RAW 264.7 cells maintained in RPMI-1640 medium, but not α-MEM, which was also supported by mouse bone marrow-derived macrophages, although they were less sensitive to LPS than RAW 264.7 cells. LPS augmented the expression of nuclear factor of activated T-cells (NFATc1) as a key transcription factor of osteoclastogenesis in cells maintained in RPMI-1640 medium, but reduced it in cells maintained in α-MEM. A high concentration of LPS was cytotoxic against cells maintained in α-MEM. Glutathione exclusively present in RPMI-1640 medium prevented LPS-induced cell death in α-MEM and augmented LPS-induced NFATc1 expression, followed by enhanced LPS-induced OC formation. LPS induced higher generation of reactive oxygen species in α-MEM than RPMI-1640 medium. An antioxidant enhanced LPS-induced OC formation, whereas a pro-oxidant reduced it. Taken together, redox balance in the culture condition was suggested to regulate in vitro LPS-induced OC formation.
|
| Volume |
21(2)
|
| Pages |
194-202
|
| Published |
2015-2-1
|
| DOI |
10.1177/1753425914524242
|
| PII |
1753425914524242
|
| PMID |
24595208
|
| MeSH |
Animals
Cell Death
Cell Differentiation
Cell Line
Culture Media / metabolism*
Glutathione / metabolism*
In Vitro Techniques
Lipopolysaccharides / metabolism*
Macrophages / physiology*
Male
Mice
Mice, Inbred C57BL
NF-kappa B / metabolism
NFATC Transcription Factors / metabolism
Osteoclasts / physiology*
Oxidation-Reduction
Reactive Oxygen Species / metabolism
|
| IF |
2.298
|
| Times Cited |
6
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOCHEMISTRY & MOLECULAR BIOLOGY
MICROBIOLOGY
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
