論文 - 詳細
| RRC ID | 44954 |
|---|---|
| 著者 | Hayashida C, Ito J, Nakayachi M, Okayasu M, Ohyama Y, Hakeda Y, Sato T. |
| タイトル | Osteocytes produce interferon-β as a negative regulator of osteoclastogenesis. |
| ジャーナル | J Biol Chem |
| Abstract |
Osteoclastogenesis is controlled by osteocytes; osteocytic osteoclastogenesis regulatory molecules are largely unknown. We searched for such factors using newly developed culture methods. Our culture system mimics the three-dimensional cellular structure of bone, consisting of collagen gel-embedded osteocytic MLO-Y4 cells, stromal ST2 cells on the gel as bone lining cells, and bone marrow cells. The gel-embedded MLO-Y4 cells inhibited the osteoclastogenesis induced by 1,25(OH)2D3 without modulating receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) production by ST2 cells, despite MLO-Y4 cells supported osteoclastogenesis in the absence of ST2 cells. In the bone marrow cell culture, the conditioned medium from MLO-Y4 cells decreased the capability of osteoclastic differentiation from the cells induced by macrophage colony-stimulating factor. This decreased capability was concomitant with an increase in protein kinase R mRNA expression and an inhibition of c-Fos translation. These changes were partially normalized by the simultaneous addition of an anti-interferon (IFN)-β neutralizing antibody to MLO-Y4 cell conditioned medium. To study primary osteocytes, we prepared non-osteocytic cell-free osteocyte-enriched bone fragments (OEBFs). When osteoclast precursors were induced by macrophage colony-stimulating factor in the presence of OEBFs, the generated cells exhibited a diminished capacity for osteoclastogenesis. OEBFs prepared from OPG-knock-out mice exhibited a similar effect, indicating OPG-independent inhibition. The addition of anti-IFN-β neutralizing antibody during the co-culture with OEBFs partially recovered the osteoclastogenic potential of the generated cells. The MLO-Y4 cells and OEBFs expressed IFN-β mRNA. Although osteocytic RANKL is known to be important for osteoclastogenesis, our data suggest that osteocytes also produce IFN-β as an inhibitor of osteoclastogenesis. |
| 巻・号 | 289(16) |
| ページ | 11545-11555 |
| 公開日 | 2014-4-18 |
| DOI | 10.1074/jbc.M113.523811 |
| PII | S0021-9258(20)48368-1 |
| PMID | 24610813 |
| PMC | PMC4036289 |
| MeSH | Animals Antibodies, Neutralizing / pharmacology Calcitriol / pharmacology Cell Differentiation / drug effects Cell Differentiation / physiology* Cells, Cultured Interferon-beta / antagonists & inhibitors Interferon-beta / genetics Interferon-beta / metabolism* Macrophage Colony-Stimulating Factor / genetics Macrophage Colony-Stimulating Factor / metabolism* Male Mice Mice, Knockout Osteoclasts / cytology Osteoclasts / metabolism* Osteocytes / cytology Osteocytes / metabolism* Osteoprotegerin / genetics Osteoprotegerin / metabolism RANK Ligand / genetics RANK Ligand / metabolism* |
| IF | 4.238 |
| 引用数 | 21 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |