RRC ID 44957
著者 Hirata N, Nakagawa M, Fujibayashi Y, Yamauchi K, Murata A, Minami I, Tomioka M, Kondo T, Kuo TF, Endo H, Inoue H, Sato SI, Ando S, Kawazoe Y, Aiba K, Nagata K, Kawase E, Chang YT, Suemori H, Eto K, Nakauchi H, Yamanaka S, Nakatsuji N, Ueda K, Uesugi M.
タイトル A chemical probe that labels human pluripotent stem cells.
ジャーナル Cell Rep
Abstract A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.
巻・号 6(6)
ページ 1165-1174
公開日 2014-3-27
DOI 10.1016/j.celrep.2014.02.006
PII S2211-1247(14)00085-0
PMID 24613351
MeSH Animals Fluorescent Dyes / chemistry* HEK293 Cells Humans Induced Pluripotent Stem Cells / chemistry* Induced Pluripotent Stem Cells / cytology* Mice Microscopy, Fluorescence / methods Molecular Probes / chemistry* Pluripotent Stem Cells / chemistry* Pluripotent Stem Cells / cytology*
IF 8.109
引用数 28
WOS 分野 CELL BIOLOGY
リソース情報
ヒト・動物細胞 10T1/2(RCB0247)