論文 - 詳細
| RRC ID | 44957 |
|---|---|
| 著者 | Hirata N, Nakagawa M, Fujibayashi Y, Yamauchi K, Murata A, Minami I, Tomioka M, Kondo T, Kuo TF, Endo H, Inoue H, Sato SI, Ando S, Kawazoe Y, Aiba K, Nagata K, Kawase E, Chang YT, Suemori H, Eto K, Nakauchi H, Yamanaka S, Nakatsuji N, Ueda K, Uesugi M. |
| タイトル | A chemical probe that labels human pluripotent stem cells. |
| ジャーナル | Cell Rep |
| Abstract |
A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents. |
| 巻・号 | 6(6) |
| ページ | 1165-1174 |
| 公開日 | 2014-3-27 |
| DOI | 10.1016/j.celrep.2014.02.006 |
| PII | S2211-1247(14)00085-0 |
| PMID | 24613351 |
| MeSH | Animals Fluorescent Dyes / chemistry* HEK293 Cells Humans Induced Pluripotent Stem Cells / chemistry* Induced Pluripotent Stem Cells / cytology* Mice Microscopy, Fluorescence / methods Molecular Probes / chemistry* Pluripotent Stem Cells / chemistry* Pluripotent Stem Cells / cytology* |
| IF | 8.109 |
| 引用数 | 28 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 24 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |