Reference - Detail
|Author||Sakai T, Mashima H, Yamada Y, Goto T, Sato W, Dohmen T, Kamada K, Yoshioka M, Uchinami H, Yamamoto Y, Ohnishi H.|
|Title||The roles of interferon regulatory factors 1 and 2 in the progression of human pancreatic cancer.|
OBJECTIVE:Pancreatic cancer is one of the most malignant diseases worldwide. Interferon regulatory factor (IRF) 1 and IRF2 function as a tumor suppressor and oncoprotein, respectively, in several types of cancers. We investigated whether IRF1 and IRF2 are involved in the progression of pancreatic cancer.
METHODS:We examined the expressions of IRF1 and IRF2 in pancreatic cancer specimens and analyzed the association with clinicopathologic features. We evaluated the biological effects of IRF1 and IRF2 using a pancreatic cancer cell line.
RESULTS:The expression levels of IRF1 and IRF2 were decreased and increased, respectively, in the pancreatic cancer cells compared with those observed in the paired normal areas. A higher expression of IRF1 was associated with better features of tumor differentiation, infiltration depth, tumor size, and survival, whereas that of IRF2 was associated with a worse feature of tumor infiltration depth. Interferon regulatory factor 2-overexpressing PANC-1 cells exhibited an increase in cell growth, less apoptotic features, and chemoresistance to gemcitabine treatment. In contrast, IRF1-overexpressing cells exhibited the opposite characteristics.
CONCLUSIONS:Interferon regulatory factors 1 and 2 may regulate the progression of pancreatic cancer by functioning as an antioncoprotein and oncoprotein, respectively. These molecules may serve as potential targets of therapy.
|MeSH||Adult Aged Aged, 80 and over Antimetabolites, Antineoplastic / pharmacology Apoptosis / drug effects Caco-2 Cells Cell Line, Tumor Cell Proliferation / drug effects Deoxycytidine / analogs & derivatives Deoxycytidine / pharmacology Disease Progression Female Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Interferon Regulatory Factor-1 / genetics Interferon Regulatory Factor-1 / metabolism* Interferon Regulatory Factor-2 / genetics Interferon Regulatory Factor-2 / metabolism* Kaplan-Meier Estimate Male Middle Aged Mutation Pancreatic Neoplasms / genetics Pancreatic Neoplasms / metabolism* Pancreatic Neoplasms / pathology Reverse Transcriptase Polymerase Chain Reaction|
|WOS Category||GASTROENTEROLOGY & HEPATOLOGY|
|Human and Animal Cells|