RRC ID 44979
Author Yukawa M, Akiyama T, Franke V, Mise N, Isagawa T, Suzuki Y, Suzuki MG, Vlahovicek K, Abe K, Aburatani H, Aoki F.
Title Genome-wide analysis of the chromatin composition of histone H2A and H3 variants in mouse embryonic stem cells.
Journal PLoS One
Abstract Genome-wide distribution of the majority of H2A and H3 variants (H2A, H2AX, H2AZ, macroH2A, H3.1, H3.2 and H3.3) was simultaneously investigated in mouse embryonic stem cells by chromatin immunoprecipitation sequencing. Around the transcription start site, histone variant distribution differed between genes possessing promoters of high and low CpG density, regardless of their expression levels. In the intergenic regions, regulatory elements were enriched in H2A.Z and H3.3, whereas repeat elements were abundant in H2A and macroH2A, and H3.1, respectively. Analysis of H2A and H3 variant combinations composing nucleosomes revealed that the H2A.Z and H3.3 combinations were present at a higher frequency throughout the genome than the other combinations, suggesting that H2A.Z and H3.3 associate preferentially with each other to comprise the nucleosomes independently of genome region. Finally, we found that chromatin was unstable only in regions where it was enriched in both H2A.Z and H3.3, but strongly quantified stable in regions in which only H3.3 was abundant. Therefore, histone variant composition is an important determinant of chromatin structure, which is associated with specific genomic functions.
Volume 9(3)
Pages e92689
Published 2014-1-1
DOI 10.1371/journal.pone.0092689
PII PONE-D-13-48426
PMID 24658136
PMC PMC3962432
MeSH Animals Cell Line Chromatin / metabolism* Chromatin Assembly and Disassembly Chromatin Immunoprecipitation CpG Islands Embryonic Stem Cells / metabolism* Genetic Variation* Genome-Wide Association Study Histones / genetics* Histones / metabolism* Mice Promoter Regions, Genetic Protein Binding Regulatory Elements, Transcriptional Repetitive Sequences, Nucleic Acid Transcription Initiation Site
IF 2.74
Times Cited 13
Human and Animal Cells