論文 - 詳細
| RRC ID | 44979 |
|---|---|
| 著者 | Ozaki Y, Takagi Y, Saito Y, Mori H, Hara M. |
| タイトル | Purification of porcine hair keratin subunits and their immobilization for use as cell culture substrates. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
We purified both the type I subunit and type II subunit of porcine hair keratin and compared their ability to form a uniform film of reconstituted keratin on a culture plate, and their effect on a model of neural cells. We observed the surface of the keratin-immobilized plate using a scanning electron microscope (SEM) and measured water contact angles to characterize the surface. We cultured PC12 cells on plates on which crude keratin, the type I subunit, or the type II subunit were immobilized. The water contact angles were slightly different from each other. The cells proliferated well on all three keratin-immobilized plates. The type II subunit showed a tendency to inhibit the differentiation of PC12 cells significantly as an extension of the cell shapes and neurite outgrowth in comparison with the crude extract and the type I subunit. The type I subunit and the type II subunit showed slight differences in cell differentiation, but not in cell proliferation. |
| 巻・号 | 77(9) |
| ページ | 1894-900 |
| 公開日 | 2013-1-1 |
| DOI | 10.1271/bbb.130339 |
| PII | DN/JST.JSTAGE/bbb/130339 |
| PMID | 24018666 |
| MeSH | Amino Acid Sequence Animals Cell Culture Techniques / methods* Cell Differentiation / drug effects Cell Proliferation / drug effects Hair / chemistry* Immobilized Proteins / chemistry* Immobilized Proteins / isolation & purification Immobilized Proteins / metabolism* Immobilized Proteins / pharmacology Keratins / chemistry* Keratins / isolation & purification Keratins / metabolism* Keratins / pharmacology Keratins, Type I / chemistry Keratins, Type I / isolation & purification Keratins, Type I / metabolism Keratins, Type I / pharmacology Keratins, Type II / chemistry Keratins, Type II / isolation & purification Keratins, Type II / metabolism Keratins, Type II / pharmacology Molecular Sequence Data PC12 Cells Protein Stability Rats Surface Properties Swine* |
| IF | 2.74 |
| 引用数 | 13 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |