論文 - 詳細
| RRC ID | 44992 |
|---|---|
| 著者 | Sawazaki R, Ishihara T, Usui S, Hayashi E, Tahara K, Hoshino T, Higuchi A, Nakamura S, Tsubota K, Mizushima T. |
| タイトル | Diclofenac protects cultured human corneal epithelial cells against hyperosmolarity and ameliorates corneal surface damage in a rat model of dry eye. |
| ジャーナル | Invest Ophthalmol Vis Sci |
| Abstract |
PURPOSE:Dry eye syndrome (DES) is characterized by an increase in tear osmolarity and induction of the expression and nuclear localization of an osmoprotective transcription factor (nuclear factor of activated T-cells 5 [NFAT5]) that plays an important role in providing protection against hyperosmotic tears. In this study, we screened medicines already in clinical use with a view of finding compounds that protect cultured human corneal epithelial cells against hyperosmolarity-induced cell damage. METHODS:Viable cell number was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and cellular NFAT5 level was measured by immunoblotting. The rat model for DES was developed by removal of the lacrimal glands, with an assessment of corneal surface damage based on levels of fluorescein staining and epithelial apoptosis. RESULTS:Some nonsteroidal anti-inflammatory drugs (NSAIDs), including diclofenac sodium (diclofenac), were identified during the screening procedure. These NSAIDs were able to suppress hyperosmolarity-induced apoptosis and cell growth arrest. In contrast, other NSAIDs, including bromfenac sodium (bromfenac), did not exert such a protective action. Treatment of cells with diclofenac, but not bromfenac, stimulated both the nuclear localization and expression of NFAT5 under hyperosmotic conditions. In the rat model for DES, topical administration of diclofenac (but not bromfenac) to eyes reduced corneal surface damage without affecting the volume of tear fluid. CONCLUSIONS:Diclofenac appears to protect cells against hyperosmolarity-induced cell damage and NFAT5 would play an important role in this protective action. The findings reported here may also indicate that the topical administration of diclofenac to eyes may be therapeutically beneficial for DES patients. |
| 巻・号 | 55(4) |
| ページ | 2547-56 |
| 公開日 | 2014-4-21 |
| DOI | 10.1167/iovs.13-13850 |
| PII | iovs.13-13850 |
| PMID | 24677100 |
| MeSH | Animals Anti-Inflammatory Agents, Non-Steroidal / therapeutic use Cells, Cultured Diclofenac / therapeutic use* Disease Models, Animal Dry Eye Syndromes / drug therapy* Dry Eye Syndromes / metabolism Dry Eye Syndromes / pathology Epithelium, Corneal / drug effects* Epithelium, Corneal / metabolism Epithelium, Corneal / pathology Male Osmotic Pressure / drug effects Rats Rats, Sprague-Dawley Transcription Factors / metabolism Treatment Outcome |
| IF | 3.47 |
| 引用数 | 21 |
| WOS 分野 | OPHTHALMOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |