RRC ID 45002
Author Qi H, Abe N, Zhu B, Murata Y, Nakamura Y.
Title (-)-Epigallocatechin-3-gallate ameliorates photodynamic therapy responses in an in vitro T lymphocyte model.
Journal Phytother Res
Abstract (-)-Epigallocatechin-3-gallate (EGCG), the most abundant polyphenolic constituent in green tea, is known as a powerful antioxidant but concomitantly possesses a prooxidant property. We investigated the effect of EGCG on phloxine B (PhB)-induced photocytotoxicity in human T lymphocytic leukemia Jurkat cells. EGCG significantly potentiated PhB-induced photocytotoxic effects, including the inhibition of cell proliferation, DNA fragmentation, and caspase-3 activity induction in Jurkat cells. Catalase attenuated the enhanced cytotoxicity by EGCG, suggesting the involvement of extracellularly produced hydrogen peroxide. Indeed, EGCG significantly enhanced extracellular hydrogen peroxide formation induced by photo-irradiated PhB. The EGCG also enhanced intracellular reactive oxygen species accumulation, c-Jun N-terminal kinase (JNK) phosphorylation, and interferon-γ (IFN-γ) gene expression, all of which are involved in PhB-induced apoptosis. Taken together, our data suggest that EGCG is capable of potentiating photodynamic therapy responses, presumably through the intracellular oxidative stress-sensitive JNK/IFN-γ pathway by exogenous hydrogen peroxide formation.
Volume 28(10)
Pages 1486-91
Published 2014-10-1
DOI 10.1002/ptr.5152
PMID 24700514
MeSH Apoptosis / drug effects Caspase 3 / metabolism Catalase / metabolism Catechin / analogs & derivatives* Catechin / pharmacology Cell Proliferation / drug effects DNA Fragmentation Humans Hydrogen Peroxide / metabolism Interferon-gamma / metabolism JNK Mitogen-Activated Protein Kinases / metabolism Jurkat Cells / drug effects* Photochemotherapy* Reactive Oxygen Species / metabolism
IF 4.087
Times Cited 4
WOS Category PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL
Resource
Human and Animal Cells Jurkat