論文 - 詳細
| RRC ID | 45002 |
|---|---|
| 著者 | Qi H, Abe N, Zhu B, Murata Y, Nakamura Y. |
| タイトル | (-)-Epigallocatechin-3-gallate ameliorates photodynamic therapy responses in an in vitro T lymphocyte model. |
| ジャーナル | Phytother Res |
| Abstract |
(-)-Epigallocatechin-3-gallate (EGCG), the most abundant polyphenolic constituent in green tea, is known as a powerful antioxidant but concomitantly possesses a prooxidant property. We investigated the effect of EGCG on phloxine B (PhB)-induced photocytotoxicity in human T lymphocytic leukemia Jurkat cells. EGCG significantly potentiated PhB-induced photocytotoxic effects, including the inhibition of cell proliferation, DNA fragmentation, and caspase-3 activity induction in Jurkat cells. Catalase attenuated the enhanced cytotoxicity by EGCG, suggesting the involvement of extracellularly produced hydrogen peroxide. Indeed, EGCG significantly enhanced extracellular hydrogen peroxide formation induced by photo-irradiated PhB. The EGCG also enhanced intracellular reactive oxygen species accumulation, c-Jun N-terminal kinase (JNK) phosphorylation, and interferon-γ (IFN-γ) gene expression, all of which are involved in PhB-induced apoptosis. Taken together, our data suggest that EGCG is capable of potentiating photodynamic therapy responses, presumably through the intracellular oxidative stress-sensitive JNK/IFN-γ pathway by exogenous hydrogen peroxide formation. |
| 巻・号 | 28(10) |
| ページ | 1486-91 |
| 公開日 | 2014-10-1 |
| DOI | 10.1002/ptr.5152 |
| PMID | 24700514 |
| MeSH | Apoptosis / drug effects Caspase 3 / metabolism Catalase / metabolism Catechin / analogs & derivatives* Catechin / pharmacology Cell Proliferation / drug effects DNA Fragmentation Humans Hydrogen Peroxide / metabolism Interferon-gamma / metabolism JNK Mitogen-Activated Protein Kinases / metabolism Jurkat Cells / drug effects* Photochemotherapy* Prohibitins Reactive Oxygen Species / metabolism |
| IF | 4.087 |
| 引用数 | 4 |
| WOS 分野 | PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat |