RRC ID 45021
著者 Ahadian S, Ramón-Azcón J, Estili M, Obregón R, Shiku H, Matsue T.
タイトル Facile and rapid generation of 3D chemical gradients within hydrogels for high-throughput drug screening applications.
ジャーナル Biosens Bioelectron
Abstract We propose a novel application of dielectrophoresis (DEP) to make three-dimensional (3D) methacrylated gelatin (GelMA) hydrogels with gradients of micro- and nanoparticles. DEP forces were able to manipulate micro- and nanoparticles of different sizes and materials (i.e., C2C12 myoblasts, polystyrene beads, gold microparticles, and carbon nanotubes) within GelMA hydrogels in a rapid and facile way and create 3D gradients of these particles in a microchamber. Immobilization of drugs, such as fluorescein isothiocyanate-dextran (FITC-dextran) and 6-hydroxydopamine (6-OHDA), on gold microparticles allowed us to investigate the high-throughput release of these drugs from GelMA-gold microparticle gradient systems. The latter gradient constructs were incubated with C2C12 myoblasts for 24h to examine the cell viability through the release of 6-OHDA. The drug was released from the microparticles in a gradient manner, inducing a cell viability gradient. This novel approach to create 3D chemical gradients within hydrogels is scalable to any arbitrary length scale. It is useful for making anisotropic biomimetic materials and high-throughput platforms to investigate cell-microenvironment interactions in a rapid, simple, cost-effective, and reproducible manner.
巻・号 59
ページ 166-73
公開日 2014-9-15
DOI 10.1016/j.bios.2014.03.031
PII S0956-5663(14)00212-7
PMID 24727602
MeSH Animals Biosensing Techniques / methods Cell Survival / drug effects Dextrans / chemistry Drug Evaluation, Preclinical / methods* Electrophoresis / methods Fluorescein-5-isothiocyanate / analogs & derivatives Fluorescein-5-isothiocyanate / chemistry High-Throughput Screening Assays / methods* Hydrogels / chemistry* Mice Oxidopamine / chemistry Oxidopamine / pharmacology Sympatholytics / chemistry Sympatholytics / pharmacology
IF 10.257
引用数 18
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOPHYSICS CHEMISTRY, ANALYTICAL ELECTROCHEMISTRY NANOSCIENCE & NANOTECHNOLOGY
リソース情報
ヒト・動物細胞 C2C12(RCB0987)