論文 - 詳細
| RRC ID | 45021 |
|---|---|
| 著者 | Ahadian S, Ramón-Azcón J, Estili M, Obregón R, Shiku H, Matsue T. |
| タイトル | Facile and rapid generation of 3D chemical gradients within hydrogels for high-throughput drug screening applications. |
| ジャーナル | Biosens Bioelectron |
| Abstract |
We propose a novel application of dielectrophoresis (DEP) to make three-dimensional (3D) methacrylated gelatin (GelMA) hydrogels with gradients of micro- and nanoparticles. DEP forces were able to manipulate micro- and nanoparticles of different sizes and materials (i.e., C2C12 myoblasts, polystyrene beads, gold microparticles, and carbon nanotubes) within GelMA hydrogels in a rapid and facile way and create 3D gradients of these particles in a microchamber. Immobilization of drugs, such as fluorescein isothiocyanate-dextran (FITC-dextran) and 6-hydroxydopamine (6-OHDA), on gold microparticles allowed us to investigate the high-throughput release of these drugs from GelMA-gold microparticle gradient systems. The latter gradient constructs were incubated with C2C12 myoblasts for 24h to examine the cell viability through the release of 6-OHDA. The drug was released from the microparticles in a gradient manner, inducing a cell viability gradient. This novel approach to create 3D chemical gradients within hydrogels is scalable to any arbitrary length scale. It is useful for making anisotropic biomimetic materials and high-throughput platforms to investigate cell-microenvironment interactions in a rapid, simple, cost-effective, and reproducible manner. |
| 巻・号 | 59 |
| ページ | 166-73 |
| 公開日 | 2014-9-15 |
| DOI | 10.1016/j.bios.2014.03.031 |
| PII | S0956-5663(14)00212-7 |
| PMID | 24727602 |
| MeSH | Animals Biosensing Techniques / methods Cell Survival / drug effects Dextrans / chemistry Drug Evaluation, Preclinical / methods* Electrophoresis / methods Fluorescein-5-isothiocyanate / analogs & derivatives Fluorescein-5-isothiocyanate / chemistry High-Throughput Screening Assays / methods* Hydrogels / chemistry* Mice Oxidopamine / chemistry Oxidopamine / pharmacology Sympatholytics / chemistry Sympatholytics / pharmacology |
| IF | 10.257 |
| 引用数 | 18 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOPHYSICS CHEMISTRY, ANALYTICAL ELECTROCHEMISTRY NANOSCIENCE & NANOTECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |