Reference - Detail
RRC ID | 45034 |
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Author | Shi M, Yang Y, Guan D, Zhang Y, Zhang Z. |
Title | Bioactivity of the crude polysaccharides from fermented soybean curd residue by Flammulina velutipes. |
Journal | Carbohydr Polym |
Abstract |
The solid-state fermentation, reusing soybean curd residue (SCR) as a solid substrate, was conducted for producing polysaccharides by Flammulina velutipes (F. velutipes). The optimal fermentation conditions were 74.5% of moisture content, 9.69 of inoculum size and 30.27 of C/N ratio by response surface methodology. 59.15 mg/g of polysaccharides were obtained. F. velutipes polysaccharides were subsequently extracted from fermented SCR by ultrasonic assisted extraction. The optimal extract conditions were 30 min, 80 °C, 150 W and 20 of water to solid ratio and 106.74 mg/g of polysaccharides were obtained. Furthermore, the antioxidant and the immunomodulatory activities of polysaccharides were assessed. The results showed that polysaccharides exhibited a strong DPPH radical scavenging activity, SOD-like activity, stimulatory the proliferation of the macrophage, the production of nitric oxide, phagocytosis and the protection on Doxorubicin damage. These could lay the foundation for changing SCR into a nutritious functional food or a food additive. |
Volume | 89(4) |
Pages | 1268-76 |
Published | 2012-8-1 |
DOI | 10.1016/j.carbpol.2012.04.047 |
PII | S0144-8617(12)00387-6 |
PMID | 24750941 |
MeSH | Animals Cell Line Cell Proliferation / drug effects Flammulina* / chemistry Flammulina* / metabolism Free Radical Scavengers* / chemistry Free Radical Scavengers* / isolation & purification Free Radical Scavengers* / metabolism Free Radical Scavengers* / pharmacology Fungal Polysaccharides* / biosynthesis Fungal Polysaccharides* / chemistry Fungal Polysaccharides* / isolation & purification Fungal Polysaccharides* / pharmacology Macrophages / cytology Macrophages / metabolism* Mice Nitric Oxide / biosynthesis Phagocytosis / drug effects Soy Foods / microbiology* |
IF | 7.182 |
Times Cited | 38 |
WOS Category | CHEMISTRY, APPLIED POLYMER SCIENCE CHEMISTRY, ORGANIC |
Resource | |
Human and Animal Cells | RAW 264(RCB0535) |