RRC ID 45037
Author Bamba Y, Shofuda T, Kanematsu D, Nonaka M, Yamasaki M, Okano H, Kanemura Y.
Title Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics.
Journal Biochem Biophys Res Commun
Abstract Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rapid polarization and neurite extension along the radially spreading processes of the GDC90 cells, and showed migratory behavior. This method is potentially useful for detailed examination of neurites or for controlling neurites behavior for regenerative medicine.
Volume 447(4)
Pages 683-8
Published 2014-5-16
DOI 10.1016/j.bbrc.2014.04.070
PII S0006-291X(14)00712-8
PMID 24755070
MeSH Cell Differentiation / physiology Cell Line Cell Line, Tumor Cell Movement / physiology Cell Polarity / physiology Cell Shape Coculture Techniques / methods Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / physiology* Luminescent Proteins / genetics Luminescent Proteins / metabolism Membranes, Artificial Nerve Regeneration Neural Stem Cells / cytology* Neural Stem Cells / physiology* Neurites / physiology Neuroglia / cytology* Neuroglia / physiology* Recombinant Proteins / genetics Recombinant Proteins / metabolism Regenerative Medicine / methods Transfection
IF 2.985
Times Cited 8
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells