論文 - 詳細
| RRC ID | 45047 |
|---|---|
| 著者 | Ishiguro K, Ando T, Maeda O, Watanabe O, Goto H. |
| タイトル | Suppressive action of acetate on interleukin-8 production via tubulin-α acetylation. |
| ジャーナル | Immunol Cell Biol |
| Abstract |
Acetate is the major short-chain fatty acid produced by commensal bacteria in the gut and is known as a nutrient source for epithelial cells of the mucosa. Acetate also suppresses interleukin (IL)-2 production in T cells by inhibiting nuclear factor of activated T cells (NFAT) nuclear translocation via tubulin-α acetylation. Using acetylation of tubulin-α as a biomarker, we have examined the influence of acetate in the large intestine. Because of high concentrations of acetate in fecal material, tubulin-α acetylation is dominant in the proximal large intestine relative to other sections of the large intestine and is induced in epithelial cells of the colonic mucosa. Flagellin stimulation induces IL-8 production in epithelial cells and acetate suppresses this IL-8 production via tubulin-α acetylation. Flagellin stimulation activates nuclear factor-κB, CREB and AP-1, but not NFAT. Of these transcription factors, acetate specifically inhibits AP-1 activation. Acetate impairs flagellin-induced activation of the Rap1-MEK-ERK-Elk-1 pathway with acetylation of tubulin-α that is bound to Rap1, resulting in reduced expression of c-Fos, a subunit of AP-1. These findings reveal a novel action of acetate via tubulin-α acetylation in epithelial cells of the colonic mucosa. |
| 巻・号 | 92(7) |
| ページ | 624-30 |
| 公開日 | 2014-8-1 |
| DOI | 10.1038/icb.2014.31 |
| PII | icb201431 |
| PMID | 24777307 |
| MeSH | Acetates / pharmacology* Acetylation / drug effects Animals Cell Line Colon / drug effects Colon / immunology Colon / metabolism Extracellular Signal-Regulated MAP Kinases / metabolism Flagellin / immunology Humans Interleukin-8 / biosynthesis* Interleukin-8 / genetics Intestinal Mucosa / drug effects Intestinal Mucosa / immunology Intestinal Mucosa / metabolism Male Mice Protein Binding RNA, Messenger / genetics RNA, Messenger / metabolism Signal Transduction T-Lymphocyte Subsets / drug effects T-Lymphocyte Subsets / immunology T-Lymphocyte Subsets / metabolism Tubulin / metabolism* ets-Domain Protein Elk-1 / metabolism |
| IF | 3.764 |
| 引用数 | 7 |
| WOS 分野 | IMMUNOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) CW-2(RCB0778) |