| RRC ID |
45064
|
| 著者 |
Yashiro Y, Nomura Y, Kanazashi M, Noda K, Hanada N, Nakamura Y.
|
| タイトル |
Function of chemokine (CXC motif) ligand 12 in periodontal ligament fibroblasts.
|
| ジャーナル |
PLoS One
|
| Abstract |
The periodontal ligament (PDL) is one of the connective tissues located between the tooth and bone. It is characterized by rapid turnover. Periodontal ligament fibroblasts (PDLFs) play major roles in the rapid turnover of the PDL. Microarray analysis of human PDLFs (HPDLFs) and human dermal fibroblasts (HDFs) demonstrated markedly high expression of chemokine (CXC motif) ligand 12 (CXCL12) in the HPDLFs. CXCL12 plays an important role in the migration of mesenchymal stem cells (MSCs). The function of CXCL12 in the periodontal ligament was investigated in HPDLFs. Expression of CXCL12 in HPDLFs and HDFs was examined by RT-PCR, qRT-PCR and ELISA. Chemotactic ability of CXCL12 was evaluated in both PDLFs and HDFs by migration assay of MSCs. CXCL12 was also immunohistochemically examined in the PDL in vivo. Expression of CXCL12 in the HPDLFs was much higher than that in HDFs in vitro. Migration assay demonstrated that the number of migrated MSCs by HPDLFs was significantly higher than that by HDFs. In addition, the migrated MSCs also expressed CXCL12 and several genes that are familiar to fibroblasts. CXCL12 was immunohistochemically localized in the fibroblasts in the PDL of rat molars. The results suggest that PDLFs synthesize and secrete CXCL12 protein and that CXCL12 induces migration of MSCs in the PDL in order to maintain rapid turnover of the PDL.
|
| 巻・号 |
9(5)
|
| ページ |
e95676
|
| 公開日 |
2014-1-1
|
| DOI |
10.1371/journal.pone.0095676
|
| PII |
PONE-D-13-39314
|
| PMID |
24806431
|
| PMC |
PMC4012992
|
| MeSH |
Adolescent
Adult
Cell Movement / physiology*
Cells, Cultured
Chemokine CXCL12 / metabolism*
Enzyme-Linked Immunosorbent Assay
Female
Fibroblasts / metabolism*
Humans
Immunohistochemistry
Male
Periodontal Ligament / cytology*
Reverse Transcriptase Polymerase Chain Reaction
Young Adult
|
| IF |
2.74
|
| 引用数 |
8
|
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
|
| リソース情報 |
| ヒト・動物細胞 |
UCB408E6E7TERT-33(RCB2080)
UE7T-13(RCB2161)
UE6E7T-11(RCB2159) |
