| RRC ID |
45073
|
| 著者 |
Kubo Y, Aishima S, Tanaka Y, Shindo K, Mizuuchi Y, Abe K, Shirabe K, Maehara Y, Honda H, Oda Y.
|
| タイトル |
Different expression of glucose transporters in the progression of intrahepatic cholangiocarcinoma.
|
| ジャーナル |
Hum Pathol
|
| Abstract |
Glucose transporter (GLUT)-1 is expressed in malignant tumors and correlated with poor outcome in several cancers. Biliary intraepithelial neoplasia (BilIN) is considered to be a precursor or a noninvasive lesion of invasive cholangiocarcinoma. We examined GLUT-1 and GLUT-2 expression in 149 intrahepatic cholangiocarcinomas and 39 BilINs immunohistochemically and evaluated their correlation with clinicopathological findings and patient outcome in intrahepatic cholangiocarcinoma. Furthermore, we examined the role of GLUT-1 on migration and invasion of cholangiocarcinoma cells using GLUT-1 siRNA. In intrahepatic cholangiocarcinoma, GLUT-1 expression was frequently observed near the necrotic areas, whereas GLUT-2 expression tended to be observed in adenocarcinoma of large bile ducts. Compared with the GLUT-1-negative group, the GLUT-1-positive group showed significantly larger tumor size (P = .0031), poor differentiation (P < .0001), frequent lymphatic invasion (P = .0031) and lymph node metastasis (P < .0001), and high HIF-1α expression (P = .0297). GLUT-2 expression was significantly correlated with good differentiation (P = .0015), perihilar location (P < .0001), perineural invasion (P = .0049), and lymph node metastasis (P = .0248). The patients with GLUT-1-positive tumors showed poor disease related survival (P < .0001). The numbers of migrating and invading cells were significantly decreased in GLUT-1 siRNA transfectants of cholangiocarcinoma cells. Although, GLUT-1 was expressed in all grades of BilINs, GLUT-2 was expressed only in high-grade BilINs. Our results suggest that GLUT-1 expression correlates aggressive behavior and poor prognosis, and that GLUT-1 might be a therapeutic target of cholangiocarcinoma. GLUT-2 expression may be associated with cholangiocarcinogenesis of large bile duct and is a helpful marker for detecting high-grade BilIN lesions in atypical bile ducts.
|
| 巻・号 |
45(8)
|
| ページ |
1610-7
|
| 公開日 |
2014-8-1
|
| DOI |
10.1016/j.humpath.2014.03.008
|
| PII |
S0046-8177(14)00137-3
|
| PMID |
24824030
|
| MeSH |
Adult
Aged
Aged, 80 and over
Bile Duct Neoplasms / metabolism*
Bile Duct Neoplasms / pathology
Bile Ducts, Intrahepatic / metabolism*
Bile Ducts, Intrahepatic / pathology
Cell Movement
Cholangiocarcinoma / metabolism*
Cholangiocarcinoma / pathology
Disease Progression
Excitatory Amino Acid Transporter 2 / metabolism*
Female
Glucose Transporter Type 2 / metabolism*
Humans
Liver / metabolism*
Liver / pathology
Liver Neoplasms
Male
Middle Aged
Prognosis
|
| IF |
2.735
|
| 引用数 |
12
|
|
WOS 分野
|
PATHOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HuH-28(RCB1943)
RBE(RCB1292) |
