Reference - Detail
|Author||Trisonthi P, Sato A, Nishiwaki H, Tamura H.|
|Title||A new diterpene from Litsea cubeba fruits: structure elucidation and capability to induce apoptosis in HeLa cells.|
A new diterpene, identified as (+)-6-(4-hydroxy-4-methyl-2-pentenoyl)-4,6-dimethyl-5-(3-methyl-2-butenyl)-1,3-cyclohexadienecarbaldehyde (1, cubelin), was isolated from a methanol extract of Litsea cubeba fruits by normal phase column chromatography and purified by preparative HPLC. The structure elucidation was conducted by spectroscopic methods (UV, IR, ESI-TOF-MS, 1-D and 2-D NMR). Cubelin exhibited activity against HeLa cell viability and proliferation. The cells also exhibited changes in nuclear morphology which are hallmarks of apoptotic cell death. The presence of cleaved caspase-3/-7, caspase-8 and caspase-9 in the cubelin treated population indicated the potential of the compound to induce apoptosis in HeLa cells via both intrinsic and extrinsic pathways.
|MeSH||Antineoplastic Agents, Phytogenic / chemistry Antineoplastic Agents, Phytogenic / poisoning* Apoptosis / drug effects Caspase 3 / metabolism Caspase 8 / metabolism Caspase 9 / metabolism Cell Proliferation / drug effects Cell Survival / drug effects Diterpenes / chemistry* Diterpenes / pharmacology* Fruit / chemistry HeLa Cells / drug effects Humans Litsea / chemistry* Magnetic Resonance Spectroscopy Molecular Structure|
|WOS Category||CHEMISTRY, ORGANIC|
|Human and Animal Cells|