RRC ID 45131
Author Ohtsuka J, Fukumura M, Tsurudome M, Hara K, Nishio M, Kawano M, Nosaka T.
Title Vero/BC-F: an efficient packaging cell line stably expressing F protein to generate single round-infectious human parainfluenza virus type 2 vector.
Journal Gene Ther
Abstract A stable packaging cell line (Vero/BC-F) constitutively expressing fusion (F) protein of the human parainfluenza virus type 2 (hPIV2) was established for production of the F-defective and single round-infectious hPIV2 vector in a strategy for recombinant vaccine development. The F gene expression has not evoked cytostatic or cytotoxic effects on the Vero/BC-F cells and the F protein was physiologically active to induce syncytial formation with giant polykaryocytes when transfected with a plasmid expressing hPIV2 hemagglutinin-neuraminidase (HN). Transduction of the F-defective replicon RNA into the Vero/BC-F cells led to the release of the infectious particles that packaged the replicon RNA (named as hPIV2ΔF) without detectable mutations, limiting the infectivity to a single round. The maximal titer of the hPIV2ΔF was 6.0 × 10(8) median tissue culture infections dose per ml. The influenza A virus M2 gene was inserted into hPIV2ΔF, and the M2 protein was found to be highly expressed in a human lung cancer cell line after transduction. Furthermore, in vivo airway infection experiments revealed that the hPIV2ΔF was capable of delivering transgenes to hamster tracheal cells. Thus, non-transmissible or single round-infectious hPIV2 vector will be potentially applicable to human gene therapy or recombinant vaccine development.
Volume 21(8)
Pages 775-84
Published 2014-8-1
DOI 10.1038/gt.2014.55
PII gt201455
PMID 24942630
MeSH Animals Cell Line Cells, Cultured Chlorocebus aethiops Cricetinae Gene Transfer Techniques* Genetic Vectors* Humans Influenza A virus / genetics Parainfluenza Virus 2, Human / genetics* Recombinant Proteins / genetics Vaccines, Synthetic / genetics Vero Cells Viral Fusion Proteins / genetics* Viral Fusion Proteins / metabolism Viral Matrix Proteins / genetics
IF 4.128
Times Cited 1
Human and Animal Cells Vero(RCB0001)