論文 - 詳細
| RRC ID | 45131 |
|---|---|
| 著者 | Ohtsuka J, Fukumura M, Tsurudome M, Hara K, Nishio M, Kawano M, Nosaka T. |
| タイトル | Vero/BC-F: an efficient packaging cell line stably expressing F protein to generate single round-infectious human parainfluenza virus type 2 vector. |
| ジャーナル | Gene Ther |
| Abstract |
A stable packaging cell line (Vero/BC-F) constitutively expressing fusion (F) protein of the human parainfluenza virus type 2 (hPIV2) was established for production of the F-defective and single round-infectious hPIV2 vector in a strategy for recombinant vaccine development. The F gene expression has not evoked cytostatic or cytotoxic effects on the Vero/BC-F cells and the F protein was physiologically active to induce syncytial formation with giant polykaryocytes when transfected with a plasmid expressing hPIV2 hemagglutinin-neuraminidase (HN). Transduction of the F-defective replicon RNA into the Vero/BC-F cells led to the release of the infectious particles that packaged the replicon RNA (named as hPIV2ΔF) without detectable mutations, limiting the infectivity to a single round. The maximal titer of the hPIV2ΔF was 6.0 × 10(8) median tissue culture infections dose per ml. The influenza A virus M2 gene was inserted into hPIV2ΔF, and the M2 protein was found to be highly expressed in a human lung cancer cell line after transduction. Furthermore, in vivo airway infection experiments revealed that the hPIV2ΔF was capable of delivering transgenes to hamster tracheal cells. Thus, non-transmissible or single round-infectious hPIV2 vector will be potentially applicable to human gene therapy or recombinant vaccine development. |
| 巻・号 | 21(8) |
| ページ | 775-84 |
| 公開日 | 2014-8-1 |
| DOI | 10.1038/gt.2014.55 |
| PII | gt201455 |
| PMID | 24942630 |
| MeSH | Animals Cell Line Cells, Cultured Chlorocebus aethiops Cricetinae Gene Transfer Techniques* Genetic Vectors* Humans Influenza A virus / genetics Parainfluenza Virus 2, Human / genetics* Recombinant Proteins / genetics Vaccines, Synthetic / genetics Vero Cells Viral Fusion Proteins / genetics* Viral Fusion Proteins / metabolism Viral Matrix Proteins / genetics |
| IF | 4.128 |
| 引用数 | 1 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY MEDICINE, RESEARCH & EXPERIMENTAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Vero(RCB0001) |