論文 - 詳細
| RRC ID | 45140 |
|---|---|
| 著者 | Uraki S, Sugimoto K, Shiraki K, Tameda M, Inagaki Y, Ogura S, Kasai C, Nojiri K, Yoneda M, Yamamoto N, Takei Y, Nobori T, Ito M. |
| タイトル | Human β-defensin-3 inhibits migration of colon cancer cells via downregulation of metastasis-associated 1 family, member 2 expression. |
| ジャーナル | Int J Oncol |
| Abstract |
The innate immune system plays an important role as the first line of defense against many types of microbes. Accumulating reports suggest that human β-defensins (hBDs) are expressed by and have certain roles in some cancer cells. In this study, we investigated the roles of hBD-3 in colon cancer cells. The expression of hBD-3 was examined by reverse transcriptase-polymerase chain reaction analysis of colon cancer cell lines and immunohistochemical staining of colon cancer tissues. The effect of hBD-3 on proliferation of colon cancer was assessed using the MTT assay and a real-time cell analyzer, and the effect of hBD-3 on the migration of colon cancer cells was also examined. The results showed that hBD-3 is not expressed in colon cancer cells but is produced by tumor-infiltrating monocytes. Migration of colon cancer cells was significantly inhibited by hBD-3 in a dose-dependent manner, although proliferation of colon cancer cells was not affected by administration of hBD-3. Moreover, reduced expression of metastasis-associated 1 family, member 2 (MTA2) mRNA in colon cancer cells was associated with exposure to hBD-3. In conclusion, progression of colon cancer was inhibited by hBD-3 in a paracrine fashion. Therefore, hBD-3 may be a potent new agent for treating colon cancer. |
| 巻・号 | 45(3) |
| ページ | 1059-64 |
| 公開日 | 2014-9-1 |
| DOI | 10.3892/ijo.2014.2507 |
| PMID | 24969834 |
| MeSH | Cell Line, Tumor Cell Proliferation Colonic Neoplasms / genetics Colonic Neoplasms / pathology* Gene Expression Regulation, Neoplastic HT29 Cells Histone Deacetylases / genetics* Humans Neoplasm Invasiveness / genetics* Paracrine Communication RNA, Messenger / genetics Repressor Proteins / genetics* beta-Defensins / genetics* beta-Defensins / metabolism* |
| IF | 3.899 |
| 引用数 | 19 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COLO-320(RCB1193) |