RRC ID 45145
Author Yokooji T, Kameda Y, Utsumi M, Mori N, Murakami T.
Title Interaction of hydrophobic components in female urine before and after childbirth with P-glycoprotein in vitro.
Journal Pharmazie
Abstract The first urine in the morning (total 15 samples) and whole day urine (total 4 days, 17 samples) were collected from a young healthy woman during the pregnancy and lactation period, to examine the possible interactions of urine components (methanol extracts) with P-glycoprotein (P-gp) and multidrug resistance-associated proteins (MRPs). The interaction was evaluated by measuring the intracellular accumulation of rhodamine123, a P-gp substrate, in LLC-GA5-COL150 cells, or calcein, an MRP substrate, in Caco-2 cells in the absence and presence of urine components. Four first urine samples out of 12 collected before childbirth and one sample out of three collected after childbirth suppressed P-gp function significantly. The effect of pregnancy and lactation on P-gp inhibitory potencies of urine components was not observed. The whole day urine samples showed a clear circadian rhythm, in which three first urine samples in the morning out of four showed greater P-gp inhibitory potencies than other daytime samples. Interaction of urine components with MRPs was not detected. In conclusion, the concentration of endogenous P-gp inhibitor(s) was higher in the first urine in the morning, showing a clear circadian rhythm. Normal pregnancy and lactation appeared not to significantly affect the P-gp inhibitory potencies of urine components.
Volume 69(6)
Pages 430-6
Published 2014-6-1
PMID 24974576
MeSH ATP Binding Cassette Transporter, Subfamily B / genetics ATP Binding Cassette Transporter, Subfamily B / metabolism ATP Binding Cassette Transporter, Subfamily B, Member 1 / urine* ATP-Binding Cassette Transporters / biosynthesis Adult Blotting, Western Caco-2 Cells Cell Line Circadian Rhythm Female Fluoresceins / metabolism Fluorescent Dyes Humans Lactation / physiology Postpartum Period / urine* Pregnancy Rhodamine 123 Young Adult
IF 1.198
Times Cited 2
WOS Category PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY CHEMISTRY, MEDICINAL
Resource
Human and Animal Cells