RRC ID 45150
Author Garcia-Contreras R, Scougall-Vilchis RJ, Contreras-Bulnes R, Kanda Y, Nakajima H, Sakagami H.
Title Cytotoxicity and pro-inflammatory action of chemo-mechanical caries-removal agents against oral cells.
Journal In Vivo
Abstract BACKGROUND:Chemo-mechanical caries removal eliminates the outermost portion of the infected layer, leaving behind healthy dentine surfaces, with scarce dental tissue damage; however, the safety of caries solvents has not been established. The aim of the present study was to investigate the possible cytotoxicity of two popular chemo-mechanical caries removal agents.
MATERIALS AND METHODS:The cytotoxicity of Carisolv, Papacarie Duo and control vehicle solution (0.155-20% v/v) against human oral squamous cell carcinoma cells (HCS-2, HSC-3, HSC-4, Ca9-22) human gingival fibroblast (HGF), pulp (HPC) and periodontal ligament fibroblast (HPLF) was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Prostaglandin E2 (PGE2) was quantified by enzyme-linked immunosorbent assay. Changes in fine cell structure were assessed by transmission electron microscopy.
RESULTS:Carisolv exhibited neither cytotoxicity nor hormetic growth stimulation. Papacarie Duo significantly reduced the viable cell number within 30 min. HSC-4 exhibited the highest sensitivity, followed by HSC-2>HSC-3>HPLF>Ca9-22>HPC>HGF cells. Interleukin-1β (3 ng/ml) stimulated HGF, but not HPC cells to produce PGE2 in the culture medium. Papacarie Duo stimulated HGF cells to produce PGE2 in synergistic fashion with interleukin-1β.
CONCLUSION:Carisolv had acceptable biocompatibility with both normal and cancerous oral cells. On the other hand, Papacarie Duo had a rapid but slight cytotoxicity and pro-inflammatory action against oral cells, suggesting the importance of careful application of this agent.
Volume 28(4)
Pages 549-56
Published 2014-1-1
PII 28/4/549
PMID 24982221
MeSH Aminobutyrates Cell Line, Tumor Cell Survival / drug effects Dental Caries / pathology* Dental Caries / therapy Dental Cavity Preparation / adverse effects* Dental Pulp / cytology Dinoprostone / biosynthesis Fibroblasts / drug effects Fibroblasts / pathology Gels Gingiva / cytology Glutamic Acid / adverse effects Glutamic Acid / toxicity Humans Leucine / adverse effects Leucine / toxicity Lysine / adverse effects Lysine / toxicity Papain
IF 1.541
Times Cited 4
Human and Animal Cells Ca9-22(RCB1976) HSC-4(RCB1902) HSC-2(RCB1945) HSC-3(RCB1975)