RRC ID |
45156
|
Author |
Koibuchi F, Ritoh N, Aoyagi R, Funakoshi-Tago M, Tamura H.
|
Title |
Dexamethasone suppresses neurosteroid biosynthesis via downregulation of steroidogenic enzyme gene expression in human glioma GI-1 cells.
|
Journal |
Biol Pharm Bull
|
Abstract |
Emerging evidence indicates that stress hormone glucocorticoids (GC) are an important modulator of brain development and function. To investigate whether GCs modulate neurosteroid biosynthesis in neural cells, we studied the effects of GCs on steroidogenic gene expression in human glioma GI-1 cells. The GC dexamethasone (Dex) reduced steroidogenic acute regulatory protein (StAR), CYP11A1 and 3β-hydroxysteroid dehydrogenase gene expression in a dose- and GC receptor-dependent manner. In addition to its effects on steroidogenic gene expression, Dex also reduced de novo synthesis of progesterone (PROG). Furthermore, Dex inhibited all-trans retinoic acid (ATRA) and vitamin D3-induced steroidogenic gene expression and PROG production. This suggests that GC regulates steroidogenic gene expression in neural cells via cross-talk with the two fat-soluble vitamins, A and D. The relationship between the effects of GCs on neurosteroid biosynthesis and on cognitive behaviors and hippocampal neural activity is also discussed herein.
|
Volume |
37(7)
|
Pages |
1241-7
|
Published |
2014-1-1
|
DOI |
10.1248/bpb.b14-00014
|
PII |
DN/JST.JSTAGE/bpb/b14-00014
|
PMID |
24989015
|
MeSH |
Cell Culture Techniques
Cell Line, Tumor
Cholesterol Side-Chain Cleavage Enzyme / genetics*
Dexamethasone / pharmacology*
Dose-Response Relationship, Drug
Down-Regulation
Gene Expression Regulation / drug effects*
Glucocorticoids / pharmacology*
Humans
Multienzyme Complexes / genetics*
Neuroglia / drug effects
Neuroglia / enzymology
Neuroglia / metabolism
Neurotransmitter Agents / antagonists & inhibitors*
Neurotransmitter Agents / biosynthesis
Phosphoproteins / genetics*
Progesterone / antagonists & inhibitors
Progesterone / biosynthesis
Progesterone Reductase / genetics*
Receptors, Glucocorticoid / genetics
Steroid Isomerases / genetics*
|
IF |
1.863
|
Times Cited |
0
|
WOS Category
|
PHARMACOLOGY & PHARMACY
|
Resource |
Human and Animal Cells |
GI-1(RCB0763) |