論文 - 詳細
| RRC ID | 45194 |
|---|---|
| 著者 | Falk S, Ipsen DH, Appel CK, Ugarak A, Durup D, Dickenson AH, Heegaard AM. |
| タイトル | Randall Selitto pressure algometry for assessment of bone-related pain in rats. |
| ジャーナル | Eur J Pain |
| Abstract |
BACKGROUND:Deep pain is neglected compared with cutaneous sources. Pressure algometry has been validated in the clinic for assessment of bone-related pain in humans. In animal models of bone-related pain, we have validated the Randall Selitto behavioural test for assessment of acute and pathological bone pain and compared the outcome with more traditional pain-related behaviour measures. METHODS:Randall Selitto pressure algometry was performed over the anteromedial part of the tibia in naïve rats, sham-operated rats, and rats inoculated with MRMT-1 carcinoma cells in the left tibia, and the effect of morphine was investigated. Randall Selitto measures of cancer-induced bone pain were supplemented by von Frey testing, weight-bearing and limb use test. Contribution of cutaneous nociception to Randall Selitto measures were examined by local anaesthesia. RESULTS:Randall Selitto pressure algometry over the tibia resulted in reproducible withdrawal thresholds, which were dose-dependently increased by morphine. Cutaneous nociception did not contribute to Randall Selitto measures. In cancer-bearing animals, compared with sham, significant differences in pain-related behaviours were demonstrated by the Randall Selitto test on day 17 and 21 post-surgery. A difference was also demonstrated by von Frey testing, weight-bearing and limb use tests. CONCLUSION:Our results indicate that pressure applied by the Randall Selitto algometer on a region, where the bone is close to the skin, may offer a way to measure bone-related pain in animal models and could provide a supplement to the traditional behavioural tests and a means to study deep pain. |
| 巻・号 | 19(3) |
| ページ | 305-12 |
| 公開日 | 2015-3-1 |
| DOI | 10.1002/ejp.547 |
| PMID | 25057115 |
| MeSH | Analgesics, Opioid / pharmacology Animals Bone Neoplasms / physiopathology* Disease Models, Animal Dose-Response Relationship, Drug Female Morphine / pharmacology Nociceptive Pain / diagnosis* Pain Measurement / methods* Pain Threshold / drug effects Rats Rats, Sprague-Dawley Tibia / physiopathology* |
| IF | 3.492 |
| 引用数 | 6 |
| WOS 分野 | ANESTHESIOLOGY CLINICAL NEUROLOGY NEUROSCIENCES |
| リソース情報 | |
| ヒト・動物細胞 | MRMT-1(RCB2860) |