論文 - 詳細
| RRC ID | 45196 |
|---|---|
| 著者 | Isoda H, Motojima H, Onaga S, Samet I, Villareal MO, Han J. |
| タイトル | Analysis of the erythroid differentiation effect of flavonoid apigenin on K562 human chronic leukemia cells. |
| ジャーナル | Chem Biol Interact |
| Abstract |
The erythroid differentiation-inducing effect of apigenin and its derivatives on human chronic myeloid leukemia K562 has been reported but the functional group in its structure responsible for the effect has not yet been elucidated. Here, we determined the moiety responsible for the erythroid differentiation induction effect of apigenin by using different flavonoids to represent the functional groups in its structure. In addition, we compared apigenin and apigetrin, a flavonoid similar in structure to apigenin except for the glycoside in its structure. Morphological changes as well as expressions of specific markers in K562 cells treated with apigenin were compared with those treated with apigetrin, flavone, 7-hydroxyflavone, chrysin, luteolin, or naringenin. The anti-proliferative and erythroid differentiation-inducing effect of apigenin and the five flavonoids were then investigated and their effects on the α, β, and γ globin genes expressions were compared using real-time PCR. Results of the comparison between apigenin and apigetrin revealed that the glycoside part of apigetrin does not have a role in the induction of cell differentiation. Based on glycophorin A expression, the potency of the other flavonoids for induction of differentiation, was: apigenin>chrysin>flavone/7-hydroxyflavone>luteolin/naringenin. Results of the analysis of the relationship between the structure and function of the flavonoids suggest that the apigenin-induced K562 cell differentiation was due to the 2-3 double bond and hydroxyl groups in its structure. This is the first study that identified the specific functional group in apigenin that impact the erythroid differentiation effect in K562 cells. |
| 巻・号 | 220 |
| ページ | 269-77 |
| 公開日 | 2014-9-5 |
| DOI | 10.1016/j.cbi.2014.07.006 |
| PII | S0009-2797(14)00221-X |
| PMID | 25058688 |
| MeSH | Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology Apigenin / chemistry Apigenin / pharmacology* Cell Differentiation / drug effects* Erythroid Cells / cytology Erythroid Cells / drug effects* Humans K562 Cells Molecular Structure Polymerase Chain Reaction |
| IF | 3.723 |
| 引用数 | 18 |
| WOS 分野 | PHARMACOLOGY & PHARMACY TOXICOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) |