RRC ID 45210
著者 Tanaka Y, Kulkeaw K, Inoue T, Tan KS, Nakanishi Y, Shirasawa S, Sugiyama D.
タイトル Dok2 likely down-regulates Klf1 in mouse erythroleukemia cells.
ジャーナル Anticancer Res
Abstract BACKGROUND/AIM:Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. However, it still remains unclear how Dok2 functions in regulation of transcription of hematopoietic genes. To address this issue, we knocked-down Dok2 mRNA in mouse erythroleukemia cells which highly express Dok2 intrinsically.
MATERIALS AND METHODS:Mouse erythroleukemia cells were transfected with Dok2 siRNA for 24 h and gene expression of erythroid differentiation-related genes, such as GATA binding protein 1 (Gata1), Krüppel-like factor 1 (Klf1), α-globin and β-globin were assessed by real-time polymerase chain reaction.
RESULTS:Among the tested genes, expression of Klf1 exhibited a 1.94-fold increase when compared to the control 24 h after transfection. Immunocytochemistry and chromatin immunoprecipitation assays revealed that Dok2 protein localizes in the nucleus and binds to the promoter region of Klf1 gene.
CONCLUSION:Dok2 is able to control Klf1 expression by transcriptional regulation through directly binding to its promoter region.
巻・号 34(8)
ページ 4561-7
公開日 2014-8-1
PII 34/8/4561
PMID 25075100
MeSH Adaptor Proteins, Signal Transducing / physiology* Animals Cell Line, Tumor Down-Regulation Gene Expression Regulation, Leukemic Kruppel-Like Transcription Factors / genetics* Leukemia, Erythroblastic, Acute / genetics* Leukemia, Erythroblastic, Acute / pathology Mice Phosphoproteins / physiology* Promoter Regions, Genetic
IF 1.994
引用数 1
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞