論文 - 詳細
RRC ID | 45210 |
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著者 | Tanaka Y, Kulkeaw K, Inoue T, Tan KS, Nakanishi Y, Shirasawa S, Sugiyama D. |
タイトル | Dok2 likely down-regulates Klf1 in mouse erythroleukemia cells. |
ジャーナル | Anticancer Res |
Abstract |
BACKGROUND/AIM:Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. However, it still remains unclear how Dok2 functions in regulation of transcription of hematopoietic genes. To address this issue, we knocked-down Dok2 mRNA in mouse erythroleukemia cells which highly express Dok2 intrinsically. MATERIALS AND METHODS:Mouse erythroleukemia cells were transfected with Dok2 siRNA for 24 h and gene expression of erythroid differentiation-related genes, such as GATA binding protein 1 (Gata1), Krüppel-like factor 1 (Klf1), α-globin and β-globin were assessed by real-time polymerase chain reaction. RESULTS:Among the tested genes, expression of Klf1 exhibited a 1.94-fold increase when compared to the control 24 h after transfection. Immunocytochemistry and chromatin immunoprecipitation assays revealed that Dok2 protein localizes in the nucleus and binds to the promoter region of Klf1 gene. CONCLUSION:Dok2 is able to control Klf1 expression by transcriptional regulation through directly binding to its promoter region. |
巻・号 | 34(8) |
ページ | 4561-7 |
公開日 | 2014-8-1 |
PII | 34/8/4561 |
PMID | 25075100 |
MeSH | Adaptor Proteins, Signal Transducing / physiology* Animals Cell Line, Tumor Down-Regulation Gene Expression Regulation, Leukemic Kruppel-Like Transcription Factors / genetics* Leukemia, Erythroblastic, Acute / genetics* Leukemia, Erythroblastic, Acute / pathology Mice Phosphoproteins / physiology* Promoter Regions, Genetic |
IF | 1.994 |
引用数 | 1 |
WOS 分野 | ONCOLOGY |
リソース情報 | |
ヒト・動物細胞 |