論文 - 詳細
| RRC ID | 45211 |
|---|---|
| 著者 | Kim S, Tachikawa T, Fujitsuka M, Majima T. |
| タイトル | Far-red fluorescence probe for monitoring singlet oxygen during photodynamic therapy. |
| ジャーナル | J Am Chem Soc |
| Abstract |
Singlet oxygen ((1)O2), molecular oxygen in the lowest excited state, has a critical role in the cell-killing mechanism of photodynamic therapy (PDT). Although (1)O2 phosphorescence measurement has been mainly used to monitor (1)O2 formation during PDT, its intensity is far insufficient to obtain two-dimensional images of intracellular (1)O2 with the subcellular spatial resolution using the currently available near-IR detector. Here, we propose a new far-red fluorescence probe of (1)O2, namely, Si-DMA, composed of silicon-containing rhodamine and anthracene moieties as a chromophore and a (1)O2 reactive site, respectively. In the presence of (1)O2, fluorescence of Si-DMA increases 17 times due to endoperoxide formation at the anthracene moiety. With the advantage of negligible self-oxidation by photoirradiation (ΦΔ < 0.02) and selective mitochondrial localization, Si-DMA is particularly suitable for imaging (1)O2 during PDT. Among three different intracellular photosensitizers (Sens), Si-DMA could selectively detect the (1)O2 that is generated by 5-aminolevulinic acid-derived protoporphyrin IX, colocalized with Si-DMA in mitochondria. On the other hand, mitochondria-targeted KillerRed and lysosomal porphyrins could not induce fluorescence change of Si-DMA. This surprising selectivity of Si-DMA response depending on the Sens localization and photosensitization mechanism is caused by a limited intracellular (1)O2 diffusion distance (∼300 nm) and negligible generation of (1)O2 by type-I Sens, respectively. For the first time, we successfully visualized (1)O2 generated during PDT with a spatial resolution of a single mitochondrial tubule. |
| 巻・号 | 136(33) |
| ページ | 11707-15 |
| 公開日 | 2014-8-20 |
| DOI | 10.1021/ja504279r |
| PMID | 25075870 |
| MeSH | Animals Cells, Cultured Fluorescence Fluorescent Dyes / chemistry* HeLa Cells Humans Macrophages / chemistry Mice Molecular Structure Organosilicon Compounds / chemistry* Photochemotherapy* Rhodamines / chemistry* Singlet Oxygen / analysis* |
| IF | 14.612 |
| 引用数 | 135 |
| WOS 分野 | CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa RAW 264(RCB0535) |