論文 - 詳細
| RRC ID | 45212 |
|---|---|
| 著者 | Kato Y, Kaneko MK. |
| タイトル | A cancer-specific monoclonal antibody recognizes the aberrantly glycosylated podoplanin. |
| ジャーナル | Sci Rep |
| Abstract |
Podoplanin (PDPN/Aggrus/T1α), a platelet aggregation-inducing mucin-like sialoglycoprotein, is highly expressed in many cancers and normal tissues. A neutralizing monoclonal antibody (mAb; NZ-1) can block the association between podoplanin and C-type lectin-like receptor-2 (CLEC-2) and inhibit podoplanin-induced cancer metastasis, but NZ-1 reacts with podoplanin-expressing normal cells such as lymphatic endothelial cells. In this study, we established a cancer-specific mAb (CasMab) against human podoplanin. Aberrantly glycosylated podoplanin including keratan sulfate or aberrant sialylation, which was expressed in LN229 glioblastoma cells, was used as an immunogen. The newly established LpMab-2 mAb recognized both an aberrant O-glycosylation and a Thr55-Leu64 peptide from human podoplanin. Because LpMab-2 reacted with podoplanin-expressing cancer cells but not with normal cells, as shown by flow cytometry and immunohistochemistry, it is an anti-podoplanin CasMab that is expected to be useful for molecular targeting therapy against podoplanin-expressing cancers. |
| 巻・号 | 4 |
| ページ | 5924 |
| 公開日 | 2014-8-1 |
| DOI | 10.1038/srep05924 |
| PII | srep05924 |
| PMID | 25080943 |
| PMC | PMC4118152 |
| MeSH | Animals Antibodies, Monoclonal, Murine-Derived / biosynthesis Antibodies, Monoclonal, Murine-Derived / chemistry* Antineoplastic Agents / chemistry* Antineoplastic Agents / metabolism CHO Cells Cell Line, Tumor Cricetinae Cricetulus Glycosylation HEK293 Cells Humans Membrane Glycoproteins / chemistry Membrane Glycoproteins / immunology* Mice, Inbred BALB C Protein Binding Protein Processing, Post-Translational |
| IF | 3.998 |
| 引用数 | 47 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RERF-LC-AI(RCB0444) |