| RRC ID |
45216
|
| Author |
Hamaguchi R, Tanimoto T, Kuroda Y.
|
| Title |
Putative biomarker for phospholipid accumulation in cultured cells treated with phospholipidosis-inducing drugs: alteration of the phosphatidylinositol composition detected using high-performance liquid chromatography-tandem mass spectrometry.
|
| Journal |
J Chromatogr B Analyt Technol Biomed Life Sci
|
| Abstract |
We developed a high-performance liquid chromatography-tandem mass spectrometric method for phospholipid biomarker discovery and applied it to a cell-based assay system for the screening of phospholipidosis-inducing drugs. We studied the compositions of phospholipid molecules exceeding 100 species in cultured cells and found a characteristic alteration in the composition by treatment with cationic amphiphilic drugs possessing phospholipidosis-inducing potency. The compositions of phosphatidylinositol in RAW264 cells were significantly affected by the drug treatment. Similar alterations were also found in THP-1 cells. These phenomena were not observed when cells were treated with warfarin, which does not have phospholipidosis-inducing potency. Structural analysis of the altered phosphatidylinositols by a product ion scan revealed the presence of certain fatty acyl chains. Based on our findings, we proposed a prediction parameter (PP) for phospholipid accumulation calculated from the relative compositions of phosphatidylinositol species. As the dosage of imipramine (a cationic amphiphilic drug) increased, both the PP and cellular phospholipid content increased. Our results suggest that PP has potency as a biomarker for phospholipid accumulation in cells treated with drugs.
|
| Volume |
967
|
| Pages |
110-7
|
| Published |
2014-9-15
|
| DOI |
10.1016/j.jchromb.2014.07.018
|
| PII |
S1570-0232(14)00475-9
|
| PMID |
25086420
|
| MeSH |
Animals
Cell Line
Cells, Cultured
Chromatography, High Pressure Liquid / methods*
Dose-Response Relationship, Drug
Humans
Imipramine / pharmacology*
Lipidoses
Mice
Models, Biological
Phosphatidylinositols / analysis*
Phosphatidylinositols / chemistry*
Phospholipids / analysis
Phospholipids / chemistry
Principal Component Analysis
Tandem Mass Spectrometry / methods*
|
| IF |
3.004
|
| Times Cited |
5
|
|
WOS Category
|
BIOCHEMICAL RESEARCH METHODS
CHEMISTRY, ANALYTICAL
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535)
U-937
THP-1(RCB1189) |
