RRC ID 45222
著者 Kanazawa T, Nakagami G, Minematsu T, Yamane T, Huang L, Mugita Y, Noguchi H, Mori T, Sanada H.
タイトル Biological responses of three-dimensional cultured fibroblasts by sustained compressive loading include apoptosis and survival activity.
ジャーナル PLoS One
Abstract Pressure ulcers are characterized by chronicity, which results in delayed wound healing due to pressure. Early intervention for preventing delayed healing due to pressure requires a prediction method. However, no study has reported the prediction of delayed healing due to pressure. Therefore, this study focused on biological response-based molecular markers for the establishment of an assessment technology to predict delayed healing due to pressure. We tested the hypothesis that sustained compressive loading applied to three dimensional cultured fibroblasts leads to upregulation of heat shock proteins (HSPs), CD44, hyaluronan synthase 2 (HAS2), and cyclooxygenase 2 (COX2) along with apoptosis via disruption of adhesion. First, sustained compressive loading was applied to fibroblast-seeded collagen sponges. Following this, collagen sponge samples and culture supernatants were collected for apoptosis and proliferation assays, gene expression analysis, immunocytochemistry, and quantification of secreted substances induced by upregulation of mRNA and protein level. Compared to the control, the compressed samples demonstrated that apoptosis was induced in a time- and load- dependent manner; vinculin and stress fiber were scarce; HSP90α, CD44, HAS2, and COX2 expression was upregulated; and the concentrations of HSP90α, hyaluronan (HA), and prostaglandin E2 (PGE2) were increased. In addition, the gene expression of antiapoptotic Bcl2 was significantly increased in the compressed samples compared to the control. These results suggest that compressive loading induces not only apoptosis but also survival activity. These observations support that HSP90α, HA, and, PGE2 could be potential molecular markers for prediction of delayed wound healing due to pressure.
巻・号 9(8)
ページ e104676
公開日 2014-1-1
DOI 10.1371/journal.pone.0104676
PII PONE-D-14-02640
PMID 25102054
PMC PMC4125229
MeSH Animals Apoptosis* Cell Culture Techniques Cell Line Compressive Strength Cyclooxygenase 2 / biosynthesis Dinoprostone / biosynthesis Fibroblasts / cytology Fibroblasts / metabolism* Glucuronosyltransferase / biosynthesis HSP90 Heat-Shock Proteins / biosynthesis Hyaluronan Receptors / biosynthesis Hyaluronan Synthases Proto-Oncogene Proteins c-bcl-2 / biosynthesis Rats Stress, Mechanical*
IF 2.74
引用数 6
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 Rat-1(RCB1830)