RRC ID 45233
著者 Katayama N, Kato H, Taguchi Y, Tanaka A, Umeda M.
タイトル The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells.
ジャーナル Int J Mol Sci
Abstract Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway.
巻・号 15(8)
ページ 14026-43
公開日 2014-8-13
DOI 10.3390/ijms150814026
PII ijms150814026
PMID 25123134
PMC PMC4159837
MeSH Alkaline Phosphatase / metabolism Cell Differentiation / drug effects Cell Proliferation / drug effects Cells, Cultured Collagen Type I / metabolism Dental Enamel / chemistry* Flavonoids / pharmacology Humans Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / drug effects* Oligopeptides / chemical synthesis* Oligopeptides / chemistry Oligopeptides / pharmacology* Osteocalcin / metabolism
IF 4.556
引用数 5
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, MULTIDISCIPLINARY
リソース情報
ヒト・動物細胞 Yub625(HMS0038)