論文 - 詳細
| RRC ID | 45242 |
|---|---|
| 著者 | Mikami Y, Yamamoto K, Akiyama Y, Kobayashi M, Watanabe E, Watanabe N, Asano M, Shimizu N, Komiyama K. |
| タイトル | Osteogenic gene transcription is regulated via gap junction-mediated cell-cell communication. |
| ジャーナル | Stem Cells Dev |
| Abstract |
An analytical study of cell-cell communications between murine osteoblast-like MLO-A5 cells and bone marrow mesenchymal stem cell (BMSC)-like C3H10T1/2 cells was performed. C3H10T1/2 cells expressing green fluorescent protein (10T-GFP cells) were generated to enable the isolation of the BMSC-like cells from co-cultures with MLO-A5 cells. The mRNA expression levels of several osteogenic transcription factors (Runx2, Osterix, Dlx5, and Msx2) did not differ between the co-cultured and mono-cultured 10T-GFP cells, but those of alkaline phosphatase (ALP) and bone sialoprotein (BSP) were 300- to 400-fold higher in the co-cultured cells. Patch clamp and biocytin transfer assays revealed gap junction-mediated communication between co-cultured 10T-GFP and MLO-A5 cells. The addition of a gap junction inhibitor suppressed the increases in the expression levels of the ALP and BSP mRNAs in co-cultured 10T-GFP cells. Furthermore, the histone acetylation levels were higher in co-cultured 10T-GFP cells than in mono-cultured 10T-GFP cells. These results suggest that osteoblasts and BMSCs associate via gap junctions, and that gap junction-mediated signaling induces histone acetylation that leads to elevated transcription of the genes encoding ALP and BSP in BMSCs. |
| 巻・号 | 24(2) |
| ページ | 214-27 |
| 公開日 | 2015-1-15 |
| DOI | 10.1089/scd.2014.0060 |
| PMID | 25137151 |
| PMC | PMC4291207 |
| MeSH | 3T3-L1 Cells Alkaline Phosphatase / biosynthesis Alkaline Phosphatase / genetics Animals CHO Cells Cell Communication / physiology* Coculture Techniques Cricetinae Cricetulus Gap Junctions / genetics Gap Junctions / metabolism* Gene Expression Regulation / physiology HeLa Cells Humans Integrin-Binding Sialoprotein / biosynthesis Integrin-Binding Sialoprotein / genetics Mice Osteogenesis / physiology* Transcription Factors / biosynthesis Transcription Factors / genetics Transcription, Genetic / physiology* |
| IF | 3.153 |
| 引用数 | 6 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL TRANSPLANTATION HEMATOLOGY CELL & TISSUE ENGINEERING |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) 10T1/2(RCB0247) CHO HeLa |