RRC ID |
45242
|
Author |
Mikami Y, Yamamoto K, Akiyama Y, Kobayashi M, Watanabe E, Watanabe N, Asano M, Shimizu N, Komiyama K.
|
Title |
Osteogenic gene transcription is regulated via gap junction-mediated cell-cell communication.
|
Journal |
Stem Cells Dev
|
Abstract |
An analytical study of cell-cell communications between murine osteoblast-like MLO-A5 cells and bone marrow mesenchymal stem cell (BMSC)-like C3H10T1/2 cells was performed. C3H10T1/2 cells expressing green fluorescent protein (10T-GFP cells) were generated to enable the isolation of the BMSC-like cells from co-cultures with MLO-A5 cells. The mRNA expression levels of several osteogenic transcription factors (Runx2, Osterix, Dlx5, and Msx2) did not differ between the co-cultured and mono-cultured 10T-GFP cells, but those of alkaline phosphatase (ALP) and bone sialoprotein (BSP) were 300- to 400-fold higher in the co-cultured cells. Patch clamp and biocytin transfer assays revealed gap junction-mediated communication between co-cultured 10T-GFP and MLO-A5 cells. The addition of a gap junction inhibitor suppressed the increases in the expression levels of the ALP and BSP mRNAs in co-cultured 10T-GFP cells. Furthermore, the histone acetylation levels were higher in co-cultured 10T-GFP cells than in mono-cultured 10T-GFP cells. These results suggest that osteoblasts and BMSCs associate via gap junctions, and that gap junction-mediated signaling induces histone acetylation that leads to elevated transcription of the genes encoding ALP and BSP in BMSCs.
|
Volume |
24(2)
|
Pages |
214-27
|
Published |
2015-1-15
|
DOI |
10.1089/scd.2014.0060
|
PMID |
25137151
|
PMC |
PMC4291207
|
MeSH |
3T3-L1 Cells
Alkaline Phosphatase / biosynthesis
Alkaline Phosphatase / genetics
Animals
CHO Cells
Cell Communication / physiology*
Coculture Techniques
Cricetinae
Cricetulus
Gap Junctions / genetics
Gap Junctions / metabolism*
Gene Expression Regulation / physiology
HeLa Cells
Humans
Integrin-Binding Sialoprotein / biosynthesis
Integrin-Binding Sialoprotein / genetics
Mice
Osteogenesis / physiology*
Transcription Factors / biosynthesis
Transcription Factors / genetics
Transcription, Genetic / physiology*
|
IF |
3.153
|
Times Cited |
6
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
TRANSPLANTATION
HEMATOLOGY
CELL & TISSUE ENGINEERING
|
Resource |
Human and Animal Cells |
MC3T3-E1(RCB1126)
10T1/2(RCB0247)
CHO
HeLa |