RRC ID 45242
著者 Mikami Y, Yamamoto K, Akiyama Y, Kobayashi M, Watanabe E, Watanabe N, Asano M, Shimizu N, Komiyama K.
タイトル Osteogenic gene transcription is regulated via gap junction-mediated cell-cell communication.
ジャーナル Stem Cells Dev
Abstract An analytical study of cell-cell communications between murine osteoblast-like MLO-A5 cells and bone marrow mesenchymal stem cell (BMSC)-like C3H10T1/2 cells was performed. C3H10T1/2 cells expressing green fluorescent protein (10T-GFP cells) were generated to enable the isolation of the BMSC-like cells from co-cultures with MLO-A5 cells. The mRNA expression levels of several osteogenic transcription factors (Runx2, Osterix, Dlx5, and Msx2) did not differ between the co-cultured and mono-cultured 10T-GFP cells, but those of alkaline phosphatase (ALP) and bone sialoprotein (BSP) were 300- to 400-fold higher in the co-cultured cells. Patch clamp and biocytin transfer assays revealed gap junction-mediated communication between co-cultured 10T-GFP and MLO-A5 cells. The addition of a gap junction inhibitor suppressed the increases in the expression levels of the ALP and BSP mRNAs in co-cultured 10T-GFP cells. Furthermore, the histone acetylation levels were higher in co-cultured 10T-GFP cells than in mono-cultured 10T-GFP cells. These results suggest that osteoblasts and BMSCs associate via gap junctions, and that gap junction-mediated signaling induces histone acetylation that leads to elevated transcription of the genes encoding ALP and BSP in BMSCs.
巻・号 24(2)
ページ 214-27
公開日 2015-1-15
DOI 10.1089/scd.2014.0060
PMID 25137151
PMC PMC4291207
MeSH 3T3-L1 Cells Alkaline Phosphatase / biosynthesis Alkaline Phosphatase / genetics Animals CHO Cells Cell Communication / physiology* Coculture Techniques Cricetinae Cricetulus Gap Junctions / genetics Gap Junctions / metabolism* Gene Expression Regulation / physiology HeLa Cells Humans Integrin-Binding Sialoprotein / biosynthesis Integrin-Binding Sialoprotein / genetics Mice Osteogenesis / physiology* Transcription Factors / biosynthesis Transcription Factors / genetics Transcription, Genetic / physiology*
IF 3.153
引用数 6
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL TRANSPLANTATION HEMATOLOGY CELL & TISSUE ENGINEERING
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126) 10T1/2(RCB0247) CHO HeLa