Reference - Detail
| RRC ID | 45244 |
|---|---|
| Author | Abdelwahab AF, Ohyama A, Higashi T, Motoyama K, Khaled KA, Sarhan HA, Hussein AK, Arima H. |
| Title | Preparation and evaluation of polyamidoamine dendrimer conjugate with glucuronylglucosyl-β-cyclodextrin (G3) as a novel carrier for siRNA. |
| Journal | J Drug Target |
| Abstract |
Abstract In this study, we newly synthesized the polyamidoamine STARBURST dendrimer (dendrimer, generation 3: G3) conjugates with 6-O-α-(4-O-α-D-glucuronyl)-D-glucosyl-β-cyclodextrin [GUG-β-CDE (G3)] having the various degrees of substitution (DS) of GUG-β-cyclodextrin of 1.6, 3.0, 3.7, 5.0 and 8.6, and evaluated them as a siRNA transfer carrier. GUG-β-CDEs (G3) formed the positively charged and nano-order complexes with siRNA. Of the siRNA complexes with five GUG-β-CDEs (G3), the complex with GUG-β-CDE (G3, DS 3.7) showed the highest RNAi effect and cellular uptake with negligible cytotoxicity in KB cells at a charge ratio of 20. In addition, the RNAi effect and cellular uptake of the complex with GUG-β-CDE (G3, DS 3.7) were higher than those of α-CDE (G3, DS 2.4) and comparable to those of Lipofectamine™ 2000. Furthermore, the complex with GUG-β-CDE (G3, DS 3.7) possessed the endosomal escaping ability, the releasing property of siRNA in the cytoplasm and serum resistance. These results suggest that GUG-β-CDE (G3, DS 3.7) has the potential as a novel siRNA carrier. |
| Volume | 22(10) |
| Pages | 927-34 |
| Published | 2014-12-1 |
| DOI | 10.3109/1061186X.2014.950663 |
| PMID | 25148608 |
| MeSH | Dendrimers / chemistry* Endosomes / metabolism Gene Transfer Techniques Humans KB Cells Lipids / chemistry* Oligosaccharides / chemistry* RNA, Small Interfering / administration & dosage* |
| IF | 3.38 |
| Times Cited | 9 |
| WOS Category | PHARMACOLOGY & PHARMACY |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Colon-26(RCB2657) |