RRC ID |
45257
|
Author |
Ishida S, Tanaka R, Yamaguchi N, Ogata G, Mizutani T, Kawabata K, Haga H.
|
Title |
Epithelial sheet folding induces lumen formation by Madin-Darby canine kidney cells in a collagen gel.
|
Journal |
PLoS One
|
Abstract |
Lumen formation is important for morphogenesis; however, an unanswered question is whether it involves the collective migration of epithelial cells. Here, using a collagen gel overlay culture method, we show that Madin-Darby canine kidney cells migrated collectively and formed a luminal structure in a collagen gel. Immediately after the collagen gel overlay, an epithelial sheet folded from the periphery, migrated inwardly, and formed a luminal structure. The inhibition of integrin-β1 or Rac1 activity decreased the migration rate of the peripheral cells after the sheets folded. Moreover, lumen formation was perturbed by disruption of apical-basolateral polarity induced by transforming growth factor-β1. These results indicate that cell migration and cell polarity play an important role in folding. To further explore epithelial sheet folding, we developed a computer-simulated mechanical model based on the rigidity of the extracellular matrix. It indicated a soft substrate is required for the folding movement.
|
Volume |
9(8)
|
Pages |
e99655
|
Published |
2014-1-1
|
DOI |
10.1371/journal.pone.0099655
|
PII |
PONE-D-14-00556
|
PMID |
25170757
|
PMC |
PMC4149355
|
MeSH |
Animals
Cell Movement*
Collagen / chemistry*
Dogs
Epithelium / metabolism
Extracellular Matrix / chemistry*
Gels / chemistry
Integrin beta Chains / metabolism
Madin Darby Canine Kidney Cells
Models, Biological*
|
IF |
2.74
|
Times Cited |
9
|
WOS Category
|
CELL BIOLOGY
|
Resource |
Human and Animal Cells |
MDCK(RCB0995) |