論文 - 詳細
RRC ID | 45261 |
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著者 | Kuroda T, Yasuda S, Sato Y. |
タイトル | In vitro detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human induced pluripotent stem cells. |
ジャーナル | Methods Mol Biol |
Abstract |
Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are a leading candidate for regenerative medicine/cell therapies because of their capacity for pluripotency and unlimited self-renewal. However, there are significant obstacles preventing the clinical use of hPSCs. A significant safety issues is the presence of residual undifferentiated cells that have the potential to form tumors in vivo. Here, we describe the highly sensitive qRT-PCR methods for detection of residual undifferentiated cells in retinal pigment epithelial (RPE) cells derived from hiPSCs. qRT-PCR using probes and primers targeting LIN28A (LIN28) transcripts can detect residual undifferentiated cell levels as low as 0.002 % in hiPSC-derived RPE cells. We expect this method to contribute to process validation and quality control of hiPSC-derived cell therapy product. |
巻・号 | 1210 |
ページ | 183-92 |
公開日 | 2014-1-1 |
DOI | 10.1007/978-1-4939-1435-7_14 |
PMID | 25173169 |
MeSH | Cell Culture Techniques Cell Differentiation* / genetics Epithelial Cells / cytology* Epithelial Cells / metabolism Gene Expression Humans In Vitro Techniques Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Primary Cell Culture Retinal Pigment Epithelium / cytology* Retinal Pigment Epithelium / metabolism Reverse Transcriptase Polymerase Chain Reaction |
引用数 | 4 |
WOS 分野 | BIOCHEMICAL RESEARCH METHODS CELL & TISSUE ENGINEERING BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
ヒト・動物細胞 |