RRC ID 45261
著者 Kuroda T, Yasuda S, Sato Y.
タイトル In vitro detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human induced pluripotent stem cells.
ジャーナル Methods Mol Biol
Abstract Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are a leading candidate for regenerative medicine/cell therapies because of their capacity for pluripotency and unlimited self-renewal. However, there are significant obstacles preventing the clinical use of hPSCs. A significant safety issues is the presence of residual undifferentiated cells that have the potential to form tumors in vivo. Here, we describe the highly sensitive qRT-PCR methods for detection of residual undifferentiated cells in retinal pigment epithelial (RPE) cells derived from hiPSCs. qRT-PCR using probes and primers targeting LIN28A (LIN28) transcripts can detect residual undifferentiated cell levels as low as 0.002 % in hiPSC-derived RPE cells. We expect this method to contribute to process validation and quality control of hiPSC-derived cell therapy product.
巻・号 1210
ページ 183-92
公開日 2014-1-1
DOI 10.1007/978-1-4939-1435-7_14
PMID 25173169
MeSH Cell Culture Techniques Cell Differentiation* / genetics Epithelial Cells / cytology* Epithelial Cells / metabolism Gene Expression Humans In Vitro Techniques Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Primary Cell Culture Retinal Pigment Epithelium / cytology* Retinal Pigment Epithelium / metabolism Reverse Transcriptase Polymerase Chain Reaction
引用数 4
WOS 分野 BIOCHEMICAL RESEARCH METHODS CELL & TISSUE ENGINEERING BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞