| RRC ID |
45276
|
| 著者 |
Zhang B, Shimada Y, Kuroyanagi J, Nishimura Y, Umemoto N, Nomoto T, Shintou T, Miyazaki T, Tanaka T.
|
| タイトル |
Zebrafish xenotransplantation model for cancer stem-like cell study and high-throughput screening of inhibitors.
|
| ジャーナル |
Tumour Biol
|
| Abstract |
Xenotransplantation studies are important tools for studying cancer biology, especially for assaying tumor cell malignancy and providing cancer information in vivo. Cancer stem-like cells (CSCs) have been identified in many cancer types to drive tumor growth and recurrence, from "keeping" to "keep" resistant to chemotherapy and radiation therapy. In this study, we developed the xenotransplantation of CSCs derived from the leukemia and solid tumor cell lines using the zebrafish models. In adult zebrafish, we investigated that the xenografted leukemia stem cells (LSCs) from K562 cells could proliferate in vivo and keep the cancer property by re-transplantation. As for the solid tumor, these CSCs from DU145 cells (human prostate cancer) and HepG2 cells (human liver cancer) could form the tumor mass and even metastasis after xenotransplantation. In addition, the zebrafish embryos with CSC xenotransplantation could evaluate docetaxel in vivo efficiently and be available to screen the novel inhibitors by high-throughput manner. In summary, these zebrafish xenotransplantation models devote a good platform for the CSC mechanism investigation and anti-CSC inhibitor screening.
|
| 巻・号 |
35(12)
|
| ページ |
11861-9
|
| 公開日 |
2014-12-1
|
| DOI |
10.1007/s13277-014-2417-8
|
| PMID |
25209178
|
| MeSH |
Animals
Antineoplastic Agents / pharmacology*
Cell Line, Tumor
Disease Models, Animal
Drug Screening Assays, Antitumor*
Female
Hep G2 Cells
High-Throughput Screening Assays*
Humans
K562 Cells
Male
Neoplastic Stem Cells / drug effects*
Neoplastic Stem Cells / metabolism*
Neoplastic Stem Cells / transplantation
Transplantation, Heterologous
Zebrafish
|
| IF |
3.65
|
| 引用数 |
21
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2
DU145(RCB2143) |
