論文 - 詳細
| RRC ID | 45290 |
|---|---|
| 著者 | Kaneshiro S, Ebina K, Shi K, Yoshida K, Otsuki D, Yoshikawa H, Higuchi C. |
| タイトル | Bruton tyrosine kinase (Btk) suppresses osteoblastic differentiation. |
| ジャーナル | J Bone Miner Metab |
| Abstract |
The Tec family of nonreceptor tyrosine kinases has been shown to play a key role in inflammation and bone destruction. Bruton tyrosine kinase (Btk) has been the most widely studied because of its critical role in B cells. Furthermore, recent evidence has demonstrated that blocking Btk signaling is effective in ameliorating lymphoma progression and experimental arthritis. The role of Btk in osteoblastic differentiation has not been well elucidated. In this study, we demonstrated the role of Btk in osteoblastic differentiation and investigated the effects of a Btk inhibitor on osteoblastic differentiation in mouse preosteoblastic MC3T3-E1 cells, primary calvarial osteoblasts, and bone marrow stromal ST2 cells. Btk expression was detected in all three cell lines. Btk inhibition stimulated mRNA expression of osteoblastic markers (alkaline phosphatase, osteocalcin, and osterix) and promoted mineralization of the extracellular matrix. In addition, Btk knockdown caused increased mRNA expression of osteoblastic markers. Furthermore, Btk inhibition suppressed the phosphorylation of mitogen-activated protein kinase (MAPK), nuclear factor kappa B (NFκB), and protein kinase Cα (PKCα). Our results indicate that Btk may regulate osteoblastic differentiation through the MAPK, NFκB, and PKCα signaling pathways. |
| 巻・号 | 33(5) |
| ページ | 486-95 |
| 公開日 | 2015-9-1 |
| DOI | 10.1007/s00774-014-0612-8 |
| PMID | 25230818 |
| MeSH | Agammaglobulinaemia Tyrosine Kinase Alkaline Phosphatase / metabolism Animals Cell Differentiation / physiology* Cell Line Cells, Cultured Mice Mitogen-Activated Protein Kinases / metabolism NF-kappa B / metabolism Osteoblasts / metabolism* Osteoblasts / physiology* Osteocalcin / metabolism Phosphorylation / physiology Protein Kinase C-alpha / metabolism Protein-Tyrosine Kinases / metabolism* RNA, Messenger / metabolism Signal Transduction / physiology |
| IF | 2.297 |
| 引用数 | 1 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM MEDICINE, RESEARCH & EXPERIMENTAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) ST2(RCB0224) |