論文 - 詳細
| RRC ID | 45310 |
|---|---|
| 著者 | Igarashi T, Fujimoto C, Suzuki H, Ono M, Iijima O, Takahashi H, Takahashi H. |
| タイトル | Short-time exposure of hyperosmolarity triggers interleukin-6 expression in corneal epithelial cells. |
| ジャーナル | Cornea |
| Abstract |
PURPOSE:Although tear hyperosmolarity is assumed to play a major role in dry eye disease, correlation between the level of hyperosmolarity and inflammation remains unclear. The purpose of this study was to examine the effect of short-time hyperosmolarity exposure in the production of inflammatory cytokines in corneal epithelial cells in vitro. METHODS:Human corneal epithelial (HCE) cells were cultured under different osmotic conditions [310 (control), and 400-1000 mOsm]. Lactate dehydrogenase (LDH) release after short-term (10 minutes) or long-term (24 hours) hyperosmotic stress exposure was evaluated to determine HCE cell cytotoxicity. Production of inflammatory cytokines, including IL-6, IL-1β, IL-8, IL-23, and TGF-β1, due to hyperosmotic stress was also measured by enzyme-linked immunosorbent assay and semiquantitative real-time polymerase chain reaction. RESULTS:After a 24-hour culture, exposures above 700 mOsm caused all HCE cells to die, 500 and 600 mOsm damaged the cells, whereas 400 mOsm caused no morphological changes. However, there was a significant increase in the release of LDH after 24-hour cultures, even in 400 mOsm. In contrast, LDH examination showed that there was no cytotoxicity for the 10-minute exposures, even at above 800 mOsm. The significant increases in IL-6 production and mRNA expression at 700 mOsm during the short-time exposures were both dependent on the osmolarity. Other cytokines such as IL-1β, IL-8, IL-23, and TGF-β1 were not detected. CONCLUSIONS:Short-time hyperosmolarity exposure may activate IL-6 expression and production in HCE cells without cytotoxicity. These observations suggest that hyperosmolarity could cause inflammation on the ocular surface in dry eye disease. |
| 巻・号 | 33(12) |
| ページ | 1342-7 |
| 公開日 | 2014-12-1 |
| DOI | 10.1097/ICO.0000000000000256 |
| PMID | 25255138 |
| MeSH | Cells, Cultured Enzyme-Linked Immunosorbent Assay Epithelium, Corneal / drug effects* Epithelium, Corneal / metabolism Epithelium, Corneal / pathology Humans Interleukin-1beta / metabolism Interleukin-6 / metabolism* Interleukins / metabolism L-Lactate Dehydrogenase / metabolism Osmolar Concentration Osmotic Pressure / drug effects Real-Time Polymerase Chain Reaction Saline Solution, Hypertonic / toxicity* |
| IF | 2.215 |
| 引用数 | 17 |
| WOS 分野 | OPHTHALMOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |