Reference - Detail
|Author||Jiang SS, Weng DS, Wang QJ, Pan K, Zhang YJ, Li YQ, Li JJ, Zhao JJ, He J, Lv L, Pan QZ, Xia JC.|
|Title||Galectin-3 is associated with a poor prognosis in primary hepatocellular carcinoma.|
|Journal||J Transl Med|
BACKGROUND:Galectin-3, a member of the beta-galactoside-binding lectin family, is a multifunctional protein with various biological functions, including the proliferation and differentiation of tumor cells, angiogenesis, cancer progression, and metastasis. We aimed to clarify if expression of galectin-3 is related to the clinicopathological characteristics and prognosis of hepatocellular carcinoma (HCC) patients, and to explore the possible mechanisms of galectin-3 in hepatocellular carcinoma.
METHODS:First, we investigated galectin-3 mRNA and protein expression by using RT-PCR and Western blotting. Second, tissues from 165 HCC patients were used to evaluate clinicopathological characteristics and prognosis through immunohistochemical analyses. Furthermore, the functions of galectin-3 were analyzed with respect to the proliferation, cell cycle,apoptosis, migration, and invasion of HCC cell lines. Finally, we analyzed galectin-3 expression and micro-vessel density (MVD) by immunohistochemistry (IHC) to find its correlation with angiogenesis in Hepatocellular Carcinoma. Flow cytometer was used to explore apoptosis and Western-blot was used to detect the pathway proteins of apoptosis.
RESULTS:Galectin-3 showed high expression at the mRNA and protein levels in HCC cancer tissues and cell lines. Clinicopathological analyses revealed that increased expression of galectin-3 in tumors was closely associated with a poor prognosis. Galectin-3 knockdown by siRNA significantly inhibited cell growth, migration, and invasion, and induced apoptosis in HCC cells in vitro, whereas galectin-3 overexpression promoted cell growth, migration, and invasion. Correlation analysis of galectin-3 expression and micro-vessel density (MVD) showed that galectin-3 expression in tumor cells stimulates angiogenesis. The observed regulation of cell apoptosis was accompanied by the galectin-3-mediated modulation of caspase3 signaling pathways in HCC cells.
CONCLUSIONS:These data suggest that galectin-3 plays an important part in HCC progression and may serve as a prognostic factor for HCC.
|MeSH||Apoptosis Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism* Carcinoma, Hepatocellular / metabolism* Carcinoma, Hepatocellular / pathology Cell Cycle Cell Proliferation Female Galectin 3 / genetics Galectin 3 / metabolism* Humans Liver Neoplasms / metabolism* Liver Neoplasms / pathology Male Middle Aged Prognosis|
|WOS Category||MEDICINE, RESEARCH & EXPERIMENTAL|
|Human and Animal Cells|