論文 - 詳細
| RRC ID | 45313 |
|---|---|
| 著者 | Jiang SS, Weng DS, Wang QJ, Pan K, Zhang YJ, Li YQ, Li JJ, Zhao JJ, He J, Lv L, Pan QZ, Xia JC. |
| タイトル | Galectin-3 is associated with a poor prognosis in primary hepatocellular carcinoma. |
| ジャーナル | J Transl Med |
| Abstract |
BACKGROUND:Galectin-3, a member of the beta-galactoside-binding lectin family, is a multifunctional protein with various biological functions, including the proliferation and differentiation of tumor cells, angiogenesis, cancer progression, and metastasis. We aimed to clarify if expression of galectin-3 is related to the clinicopathological characteristics and prognosis of hepatocellular carcinoma (HCC) patients, and to explore the possible mechanisms of galectin-3 in hepatocellular carcinoma. METHODS:First, we investigated galectin-3 mRNA and protein expression by using RT-PCR and Western blotting. Second, tissues from 165 HCC patients were used to evaluate clinicopathological characteristics and prognosis through immunohistochemical analyses. Furthermore, the functions of galectin-3 were analyzed with respect to the proliferation, cell cycle,apoptosis, migration, and invasion of HCC cell lines. Finally, we analyzed galectin-3 expression and micro-vessel density (MVD) by immunohistochemistry (IHC) to find its correlation with angiogenesis in Hepatocellular Carcinoma. Flow cytometer was used to explore apoptosis and Western-blot was used to detect the pathway proteins of apoptosis. RESULTS:Galectin-3 showed high expression at the mRNA and protein levels in HCC cancer tissues and cell lines. Clinicopathological analyses revealed that increased expression of galectin-3 in tumors was closely associated with a poor prognosis. Galectin-3 knockdown by siRNA significantly inhibited cell growth, migration, and invasion, and induced apoptosis in HCC cells in vitro, whereas galectin-3 overexpression promoted cell growth, migration, and invasion. Correlation analysis of galectin-3 expression and micro-vessel density (MVD) showed that galectin-3 expression in tumor cells stimulates angiogenesis. The observed regulation of cell apoptosis was accompanied by the galectin-3-mediated modulation of caspase3 signaling pathways in HCC cells. CONCLUSIONS:These data suggest that galectin-3 plays an important part in HCC progression and may serve as a prognostic factor for HCC. |
| 巻・号 | 12 |
| ページ | 273 |
| 公開日 | 2014-9-27 |
| DOI | 10.1186/s12967-014-0273-3 |
| PII | s12967-014-0273-3 |
| PMID | 25260879 |
| PMC | PMC4179848 |
| MeSH | Apoptosis Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism* Carcinoma, Hepatocellular / metabolism* Carcinoma, Hepatocellular / pathology Cell Cycle Cell Proliferation Female Galectin 3 / genetics Galectin 3 / metabolism* Humans Liver Neoplasms / metabolism* Liver Neoplasms / pathology Male Middle Aged Prognosis |
| IF | 4.124 |
| 引用数 | 28 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL |
| リソース情報 | |
| ヒト・動物細胞 | HuH-7 |