RRC ID 45315
著者 Guo S, Liu HD, Liu YF, Liu L, Sun Q, Cui XJ.
タイトル Hepatoma-derived growth factor: a novel prognostic biomarker in intrahepatic cholangiocarcinoma.
ジャーナル Tumour Biol
Abstract Hepatoma-derived growth factor (HDGF) is an acidic heparin-binding protein involved in tumor progression and poor prognosis of kinds of cancers. Aimed at investigating the functions of HDGF in intrahepatic cholangiocarcinoma (IHCC), we detected the expression of HDGF by immunohistochemistry in 83 patients. Associations of HDGF with clinicopathologic features, microvascular density (MVD), and overall survival rates were further analyzed by Chi-square method, univariate or multivariate analysis. HDGF functions in IHCC proliferation, invasion, and angiogenesis were detected by MTT, transwell, and tube formation assays, respectively. As a result, we found that HDGF-positive expression rate in IHCC was 51.8 % (43/83) in IHCC. HDGF expression was significantly correlated to MVD (P = 0.031), lymphatic invasion (P = 0.030), distant metastasis (P = 0.002), and TNM stage (P = 0.037). HDGF was further identified as an independent prognostic factor in IHCC with Kaplan-Meier method (P = 0.003) and Cox-regression model (P = 0.008). Moreover, both intracellular and extracellular HDGF were proved to promote the proliferation, invasion, and angiogenesis of IHCC cell lines. In conclusion, HDGF was identified as an independent prognostic biomarker in IHCC. HDGF can promote IHCC cells progression, including proliferation, invasion, and angiogenesis, indicating HDGF could become a new promising and potential drug target of IHCC.
巻・号 36(1)
ページ 353-64
公開日 2015-1-1
DOI 10.1007/s13277-014-2651-0
PMID 25262276
MeSH Adult Aged Bile Duct Neoplasms / metabolism* Bile Duct Neoplasms / mortality Bile Duct Neoplasms / pathology Bile Ducts, Intrahepatic / metabolism* Bile Ducts, Intrahepatic / pathology Biomarkers, Tumor / metabolism* Cell Line, Tumor Cell Movement Cell Proliferation Cholangiocarcinoma / metabolism* Cholangiocarcinoma / mortality Cholangiocarcinoma / pathology Disease Progression Female Human Umbilical Vein Endothelial Cells / physiology Humans Intercellular Signaling Peptides and Proteins / metabolism* Kaplan-Meier Estimate MAP Kinase Signaling System Male Middle Aged Neoplasm Invasiveness Prognosis Proportional Hazards Models Retrospective Studies
IF 3.65
引用数 16
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 HuCCT1(RCB1960)