| RRC ID |
45317
|
| 著者 |
Munisso MC, Obika S, Yamaoka T.
|
| タイトル |
Nucleic acid delivery systems based on poly(galactosyl ureaethyl methacrylate-b-dimethylamino ethyl methacrylate) diblock copolymers.
|
| ジャーナル |
Carbohydr Polym
|
| Abstract |
Elevated low-density-lipoprotein cholesterol (LDL-C) level is a major risk factor leading to cardiovascular diseases. Therefore, since the proprotein convertase subtilisin kexin type 9 (PCSK9) regulates LDL-C receptors, it represents the appropriate target for cholesterol-lowering gene therapy. However, although delivery of antisense oligonucleotides (ODNs) is a promising therapeutic method for the treatment of several diseases, it is fundamental to develop new and efficacious carriers that transport the ODNs into the target cell in a nontoxic manner. This study reports on the synthesis, characterization and in vitro testing of a new liver specific carrier based on linear cationic diblock glycopolymer composed of galactosyl ureaethyl methacrylate (GAMA) and the primary amine-containing dimethylamino ethyl methacrylate (DMAEMA). Delivery experiments proved that the poly(galactosyl ureaethyl methacrylate -b-dimethylamino ethyl methacrylate) diblock copolymer (pGa4D47), internalized in a receptor-mediated manner, exhibited a much faster nuclear transportation than ODNs carried by pDMAEMA homopolymer or glycopolymer bearing glucose moieties.
|
| 巻・号 |
114
|
| ページ |
288-296
|
| 公開日 |
2014-12-19
|
| DOI |
10.1016/j.carbpol.2014.08.022
|
| PII |
S0144-8617(14)00791-7
|
| PMID |
25263893
|
| MeSH |
Cell Survival
Gene Transfer Techniques*
Genetic Therapy
HeLa Cells
Hep G2 Cells
Humans
Magnetic Resonance Spectroscopy
Methacrylates / chemistry
Nucleic Acids / administration & dosage*
Nucleic Acids / chemistry*
Nylons / chemistry
Polymers / chemistry*
|
| IF |
7.182
|
| 引用数 |
3
|
|
WOS 分野
|
POLYMER SCIENCE
CHEMISTRY, APPLIED
CHEMISTRY, ORGANIC
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2 |
