論文 - 詳細
| RRC ID | 45348 |
|---|---|
| 著者 | Nakayama T, Saitoh H. |
| タイトル | Tunicamycin-induced neutrophil extracellular trap (NET)-like structures in cultured human myeloid cell lines. |
| ジャーナル | Cell Biol Int |
| Abstract |
The mechanism of neutrophil extracellular trap cell death (NETosis), a regulated cell death pathway relevant to infection, autoimmunity and sepsis, is not completely known. The reason for this, at least in part, is the lack of an in vitro system that recapitulates the NETosis pathway using established human cell lines. We show that exposure of a human promyelocytic leukemia cell line HL-60 to the glycosyltransferase inhibitor tunicamycin (TM) resulted in extrusion of decompacted genomic DNAs to extracellular space, morphologically similar to NETs. Immunostaining using antibodies against NET marker proteins and bacterial trapping assay showed biochemical similarities between the TM-induced extracellular DNA structures and NETs. The NET-like structures were also generated on exposure of TM to other myeloid cell lines, such as U937 and THP-1. Thus, our findings provide an experimental setting to induce NET-like structures using cultured human myeloid cell lines, which may help our understanding of the regulation and function of NETosis. |
| 巻・号 | 39(3) |
| ページ | 355-9 |
| 公開日 | 2015-3-1 |
| DOI | 10.1002/cbin.10396 |
| PMID | 25336229 |
| MeSH | Anti-Bacterial Agents / pharmacology* Apoptosis / drug effects Cell Line, Tumor Extracellular Traps / chemistry Extracellular Traps / drug effects* Extracellular Traps / metabolism Glycosyltransferases / antagonists & inhibitors Glycosyltransferases / metabolism HL-60 Cells Humans Neutrophils / pathology Tunicamycin / pharmacology* |
| IF | 2.571 |
| 引用数 | 7 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa 293(RCB1637) |