RRC ID 45349
Author Sugimoto H, Watanabe K, Toyama T, Takahashi SS, Sugiyama S, Lee MC, Hamada N.
Title Inhibitory effects of French pine bark extract, Pycnogenol®, on alveolar bone resorption and on the osteoclast differentiation.
Journal Phytother Res
Abstract Pycnogenol(®) (PYC) is a standardized bark extract from French maritime pine (Pinus pinaster Aiton). We examined the inhibitory effects of PYC on alveolar bone resorption, which is a characteristic feature of periodontitis, induced by Porphyromonas gingivalis (P. gingivalis) and osteoclast differentiation. In rat periodontitis model, rats were divided into four groups: group A served as the non-infected control, group B was infected orally with P. gingivalis ATCC 33277, group C was administered PYC in the diet (0.025%: w/w), and group D was infected with P. gingivalis and administered PYC. Administration of PYC along with P. gingivalis infection significantly reduced alveolar bone resorption. Treatment of P. gingivalis with 1 µg/ml PYC reduced the number of viable bacterial cells. Addition of PYC to epithelial cells inhibited adhesion and invasion by P. gingivalis. The effect of PYC on osteoclast formation was confirmed by tartrate-resistant acid phosphatase staining. PYC treatment significantly inhibited osteoclast formation. Addition of PYC (1-100 µg/ml) to purified osteoclasts culture induced cell apoptosis. These results suggest that PYC may prevent alveolar bone resorption through its antibacterial activity against P. gingivalis and by suppressing osteoclastogenesis. Therefore, PYC may be useful as a therapeutic and preventative agent for bone diseases such as periodontitis.
Volume 29(2)
Pages 251-9
Published 2015-2-1
DOI 10.1002/ptr.5245
PMID 25336411
MeSH Acid Phosphatase Alveolar Bone Loss / prevention & control* Animals Anti-Bacterial Agents / pharmacology Bone and Bones / drug effects Cell Differentiation / drug effects Cell Line Cells, Cultured Epithelial Cells / drug effects Flavonoids / pharmacology* Gingiva / cytology Humans Isoenzymes Male Mice, Inbred BALB C Osteoclasts / drug effects* Periodontitis / microbiology Periodontitis / prevention & control Pinus / chemistry* Plant Bark / chemistry Plant Extracts Porphyromonas gingivalis / drug effects Rats, Sprague-Dawley Tartrate-Resistant Acid Phosphatase
IF 4.087
Times Cited 4
Human and Animal Cells MC3T3-G2/PA6(RCB1127)